[Histonet] Need help with Brain Tumor FS

[Tony Henwood]

Theresa,

The problem is probably not your technique.
Are your samples received swimming in saline?
I would be confident this is your problem.
I have just had accepted a paper entitled "Adverse Effect of Saline on
Brain Intra-Operative (Frozen Section) Histology" which should appear in
the next issue of the Journal of Histotechnology that illustrates this
artefact.
Request that your surgeons send their specimens dry on moist card or
gauze.
We also immediately fix our sections in methanol prior to rapid HE
staining, rather than air-drying. It produces a result very similar to a
paraffin section HE. Try it.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
tdobersztyn <@t> chmca.org
Sent: Friday, 14 September 2007 6:15 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Need help with Brain Tumor FS


Hello All!

Can anyone recommend a protocol for frozen section technique - to cut 
Brain tumor biopsies.

The usual ice-crystal artifact sometimes renders the Frozen Sections 
unreadable for intra-operative diagnosis.

We use a Leica UV1850 cryostat set at -21*
OCT media 
and cut our FS at 8 microns
then:
rapid stain H/E

The pathologist here tends to believe it is our technique that produces 
the artifact.

fast freezing with spray  vs slow freezing??????????

We have tried a lot of methods to eliminate this problem 

HELP!!!!!!!!!!! I know someone out there has a remedy for the headache 
this has created!!!!


Thank you all in advance!!! 
I am glad I can pose questions to others who might be dealing with the 
issues I deal with too!

Theresa
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