[Histonet] AO 860 sliding microtome and Caroline Bass
gcallis <@t> montana.edu
Thu Sep 13 16:32:42 CDT 2007
If you send me your slowmail, US postal service address, I will send you a
manual on the effective use and proper care of the microtome. This little
green booklet (collectors item!) tells how to operate the 860 along with
other AO microtomes (820 and freezing microtome). I found an extra copy in
my file this past month.
As for disposable blades in this sliding microtome, we always used a c
profile steel knife, a gigantic blade 200 mm long, for study, vibration
free operation. The clamp may not work with a disposable holder. Dorn and
Hart is a good place to get resharpening/reconditioning of the blades or do
that with a ThermoShandon whatever name now knife sharpener. This was a
superb sharpener and the microtome is a oldie but a goodie, heavy duty
finger amputator. We also used Bear Oil, a microtome oil which is still
available although Rene's suggestion for the Leica oil is ideal too.
Be careful, these microtomes are very efficient for sectioning and cutting
YOU!!! It was the first sliding microtome I used for sectioning
celloidin embedded monkey temporal mandibular joints back in 1963. It was
fun, but I did work with closed doors during its operation to avoid the
startle factor during sectioning. I kept my fingers, arms, etc intact.
Good luck with your "new" toy.
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
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