[Histonet] Acetone fixation for immunofluorescence protocols

Mauricio Avigdor bitesizellama <@t> gmail.com
Thu Sep 6 14:41:51 CDT 2007


I am having a bit of difficulty with an immunofluorescence protocol (IF). I
am using fresh tissue fixed for 10 minutes in cold acetone. Are there any
fixatives that are well suited to IF protocols? Has anyone attempted to
stain unfixed tissue?

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