[Histonet] bleeding eosin

[buck45 <@t> optonline.net]

Hi - 
I was wondering if anyone who uses xylene substitutes have had problems with the eosin bleeding out of the tissue. I don't know if it is the type and brand of eosin or something else. I have never used a substitute before and I find that the stainer needs to be changed much more that with regular xylene. I seem to get a lot of water on the slides unless I change it every other day.
Gail
Paramus, NJ