[Histonet] ERK and nuclear counterstain

[Geoff McAuliffe]

1. Nuclear fast red
2. 1% Bismark Brown in 1% acetic acid (gives pale,golden brown nuclei)
3. Basic fuchsin in 1% acetic acid, pinkish-red nuclei.
4. Scarba red.
5. Ask Vector for a recommendation.

Geoff



Melissa Mazan wrote:
> Hi all, I am doing phospho ERK staining - using the Vector MOM kit on 
> lung tissue - I want to counterstain so that I can enumerate number of 
> ERK pos nuclei with respect to other nuclei, but if I use hematoxylin 
> it obscures the signal - everything just looks dark.  Any suggestions 
> for a nuclear counterstain? Many thanks - Melissa
>
> histonet-request <@t> lists.utsouthwestern.edu wrote:
>>
>>

-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
**********************************************