[Histonet] curious about soaking paraffin blocks.

[Anna K. Schultz]

Thanks a lot for the responses and I am definitely enjoying the 'debate.'
Anna

Kaliko, Bonnie wrote:
> Well as far as animal tissue is concerned, ice and water are needed to
> hydrate the tissue in order to obtain a section.  Each tissue responds
> differently, some needing time on the ice longer then others.  
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kemlo
> Rogerson
> Sent: Thursday, October 25, 2007 10:41 AM
> To: Mike Pence; Cheri Miller; Smith Wanda; Anna K. Schultz;
> histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] curious about soaking paraffin blocks.
>
> "So if you place the block on the ice tissue up, what are you soaking,
> paraffin?"
>
> Well if soak it in ice tissue side down what are you cutting frozen
> sections? Don't understand why you'd want to potentially stick water
> back into something you've dehydrated; makes no sense. Why not just put
> blocks face down on a chiller or one of those Peltier effect thingies. 
>
>
> Kemlo Rogerson
> Pathology Manager
> DD   01934 647057 or extension 3311
> Mob 07749 754194; Pager 07659 597107;
>  
> Biology is the least of what makes someone a mother. --Oprah Winfrey 
> This e-mail is confidential and privileged. If you are not the intended
> recipient please accept my apologies; please do not disclose, copy or
> distribute information in this e-mail or take any action in reliance on
> its contents: to do so is strictly prohibited and may be unlawful.
> Please inform me that this message has gone astray before deleting it.
> Thank you for your co-operation
>  
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>   


-- 
Anna K. Schultz
SDRC Core A Research Technician
Department of Dermatology
College of Physicians and Surgeons
Columbia University
Vanderbilt Clinic 15-211
630 West 168^th Street
New York, NY 10032
Phone: (212) 305-4954