[Histonet] curious about soaking paraffin blocks.

[Kaliko, Bonnie]

Well as far as animal tissue is concerned, ice and water are needed to
hydrate the tissue in order to obtain a section.  Each tissue responds
differently, some needing time on the ice longer then others.  


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kemlo
Rogerson
Sent: Thursday, October 25, 2007 10:41 AM
To: Mike Pence; Cheri Miller; Smith Wanda; Anna K. Schultz;
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] curious about soaking paraffin blocks.

"So if you place the block on the ice tissue up, what are you soaking,
paraffin?"

Well if soak it in ice tissue side down what are you cutting frozen
sections? Don't understand why you'd want to potentially stick water
back into something you've dehydrated; makes no sense. Why not just put
blocks face down on a chiller or one of those Peltier effect thingies. 


Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
 
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