[Histonet] Warthin-Starry, microwave Dieterle substitute?

Bernice Frederick b-frederick <@t> northwestern.edu
Wed Oct 24 15:19:38 CDT 2007


All,
Sigma also sells controls as well as their kit. I've used it in the
microwave. I actually had to perfect the kit on smears from the VD clinic.
Fat chubby criiters let me tell you! At least they were highly visible.
Bernice

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Burton, Mark
Sent: Wednesday, October 24, 2007 2:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Warthin-Starry, microwave Dieterle substitute?


I would be cautious before using Nexus for Steiner stains. I would do
several
comparison runs against a manual Steiner with well known controls and
different
types of controls if you have them available. I used to purchase a
spirochete
control form Newcomer and we had a Bartonella control that we made.   

Mark Burton HTL ASCP
Brigham & Women's Hospital 
Boston, MA



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bernice
Frederick
Sent: Wednesday, October 24, 2007 2:12 PM
To: 'Rutledge, Nancy'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Warthin-Starry, microwave Dieterle substitute?

Sigma sells a modified steiner kit. Can be used standard as well as
microwave. Ventana has one for the Nexus.
Bernice

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rutledge,
Nancy
Sent: Wednesday, October 24, 2007 10:15 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Warthin-Starry, microwave Dieterle substitute?

Is there a stain currently being used to replace  Warthin Starry and/or
Dieterle?  We do VERY few and are concerned about reagent costs? 

Thanks.

Nancy Rutledge

 

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