[Histonet] curious about soaking paraffin blocks.

[Kim Merriam]

Anna,

It has been my experience that most tissues are OK if soaked for a long time; but some tissues (especially fatty ones, like brain) will swell (a lot!)  when soaked for an extended period of time.

Just be careful!

Kim
 
Kim Merriam, MA, HT(ASCP)
Cambridge, MA



----- Original Message ----
From: Anna K. Schultz <as3323 <@t> columbia.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Wednesday, October 24, 2007 11:05:37 AM
Subject: [Histonet] curious about soaking paraffin blocks.

Greetings All,
  I remember reading on here/archives that paraffin blocks should not be 
soaked or kept on ice for too long--what is the reasoning behind this?   
I am just plain curious and still fairly new to the field.  I often 
leave blocks on for a long time and this seems to help or at least not hurt.

Thanks,
Anna
-- 
Anna K. Schultz
SDRC Core A Research Technician
Department of Dermatology
College of Physicians and Surgeons
Columbia University
Vanderbilt Clinic 15-211
630 West 168^th Street
New York, NY 10032
Phone: (212) 305-4954

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