AW: [Histonet] FW: PAS/Fungus

[Gudrun Lang]

We use mayers hematoxylin 5 min as counterstain. - only nuclei-stain is
perhaps easier to distinguish from the fungi.

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Cheri
Miller
Gesendet: Donnerstag, 18. Oktober 2007 20:25
An: 'Histonet'
Betreff: [Histonet] FW: PAS/Fungus

 

 

Cheri Miller HT ASCP

Histology Supervisor
Physicians Laboratory Services, Inc.

Omaha, NE 68117

402 738 5052

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  _____  

From: Cheri Miller [mailto:cmiller <@t> physlab.com] 
Sent: Wednesday, October 17, 2007 10:38 AM
To: histonet-bounces <@t> lists.utsouthwestern.edu
Subject: PAS/Fungus

 

I have a Path that says our PAS/F is weak. His suggestion is that I do a
hematoxilyn counter instead of light green. Anyone else doing this?? Seems
to me that the counter stain won't make the PAS reaction any stronger.   Our
procedure is as follows;

.5%Periodic acid 5 mins

2 changes DI water

Schiff's 20 mins

Running water 10 mins   

Light green 1 min

95,100,100 and 3 xylene

 

I don't get any complaints from the other 6 pathologist's. Share your
wisdom. Thanks Cheri

Cheri Miller HT ASCP

Histology Supervisor
Physicians Laboratory Services, Inc.

Omaha, NE 68117

402 738 5052

PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If
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delete this email from your system.

 



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