[Histonet] FW: PAS/Fungus

[Cheri Miller]

Cheri Miller HT ASCP

Histology Supervisor
Physicians Laboratory Services, Inc.

Omaha, NE 68117

402 738 5052

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  _____  

From: Cheri Miller [mailto:cmiller <@t> physlab.com] 
Sent: Wednesday, October 17, 2007 10:38 AM
To: histonet-bounces <@t> lists.utsouthwestern.edu
Subject: PAS/Fungus

 

I have a Path that says our PAS/F is weak. His suggestion is that I do a
hematoxilyn counter instead of light green. Anyone else doing this?? Seems
to me that the counter stain won't make the PAS reaction any stronger.   Our
procedure is as follows;

.5%Periodic acid 5 mins

2 changes DI water

Schiff's 20 mins

Running water 10 mins   

Light green 1 min

95,100,100 and 3 xylene

 

I don't get any complaints from the other 6 pathologist's. Share your
wisdom. Thanks Cheri

Cheri Miller HT ASCP

Histology Supervisor
Physicians Laboratory Services, Inc.

Omaha, NE 68117

402 738 5052

PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If
you are not the addressee intended / indicated or agent responsible for
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possession of confidential and privileged information.  Any dissemination,
distribution, or copying of this e-mail is strictly prohibited.  If you have
received this message in error, please notify the sender immediately and
delete this email from your system.

 



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