[Histonet] FW: PAS/Fungus

Cheri Miller cmiller <@t> physlab.com
Thu Oct 18 13:25:19 CDT 2007


 

 

Cheri Miller HT ASCP

Histology Supervisor
Physicians Laboratory Services, Inc.

Omaha, NE 68117

402 738 5052

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  _____  

From: Cheri Miller [mailto:cmiller <@t> physlab.com] 
Sent: Wednesday, October 17, 2007 10:38 AM
To: histonet-bounces <@t> lists.utsouthwestern.edu
Subject: PAS/Fungus

 

I have a Path that says our PAS/F is weak. His suggestion is that I do a
hematoxilyn counter instead of light green. Anyone else doing this?? Seems
to me that the counter stain won't make the PAS reaction any stronger.   Our
procedure is as follows;

.5%Periodic acid 5 mins

2 changes DI water

Schiff's 20 mins

Running water 10 mins   

Light green 1 min

95,100,100 and 3 xylene

 

I don't get any complaints from the other 6 pathologist's. Share your
wisdom. Thanks Cheri

Cheri Miller HT ASCP

Histology Supervisor
Physicians Laboratory Services, Inc.

Omaha, NE 68117

402 738 5052

PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message.  If
you are not the addressee intended / indicated or agent responsible for
delivering it to the addressee, you are hereby notified that you are in
possession of confidential and privileged information.  Any dissemination,
distribution, or copying of this e-mail is strictly prohibited.  If you have
received this message in error, please notify the sender immediately and
delete this email from your system.

 



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