[Histonet] H&E problem

[Kemlo Rogerson]

"Hi All,

We are having a problem with our H&E stain.  The pathologist is seeing
circular areas on the tissues that are not staining with
	the hematoxylin(Harris), but they are picking up the Eosin.  We
are changing the first xylenes and alcohols and hoping that might help.
Any idea why this might be happening?  Thanks!  

Michele Margiotta
BMHMC
Histology Supervisor
631-654-7192"


Old chestnut this one; answers usually are poor dewaxing, sections
lifting and/ or poor fixation.

Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
 
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