[Histonet] frozen sections

[Sharon Bledsoe]

Joyce,


How does the baby powder effect your sample if you have to use 
birefringence?  Talc polarizes, I have to be careful and use 
non-powdered gloves when I section many of our animal tissue as we 
are looking for crystals that are identified by birefringence.

Do you note on the slide that you have an additive  on a frozen section?



Sharon Bledsoe










At 3:58 PM -0400 10/16/07, Weems, Joyce wrote:
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>Please the tissue in baby powder and it will eliminate the air 
>bubble. You can also add baby powder to the OCT and it will give it 
>more "body" for holding the tissue correctly for freezing. I don't 
>remember where I heard this or learned it... I just know it works! 
>Hope it works for you, j
>
>Joyce Weems
>Pathology Manager
>Saint Joseph's Hospital
>5665 Peachtree Dunwoody Rd NE
>Atlanta, GA 30342
>404-851-7376 - Phone
>404-851-7831 - Fax
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Atoska
>Gentry
>Sent: Tuesday, October 16, 2007 3:55 PM
>To: Histonet
>Subject: [Histonet] frozen sections
>
>
>hello, has anyone observed empty space surrounding tissue frozen in
>O.C.T. or similar tissue freezing medium after snap freezing? Upon
>sectioning samples shipped previously snap frozen to one of our
>researchers I noticed space surrounding various areas of the tissue.
>Does anyone know what probably caused this? Could this be a factor in
>the generation of horrible sections? I suspect the tissue may be moving
>when the blade touches it. Is there a know remedy that will not damage
>the tissue? Has anyone ever added O.C.T. to already frozen tissues? Any
>assistance will be much appreciated. Atoska
>
>--
>Atoska S. Gentry, B.S., HT(ASCP)
>Research Assistant IV
>Scott-Ritchey RSCH Center
>College of Vet. Med
>Auburn, AL 36849
>PH (334) 844-5579
>FAX (334) 844-5850
>email: gentras <@t> vetmed.auburn.edu
>
>
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