[Histonet] crazy immuno question - can you restain slides?

[Cheryl Cross]

i see - thanks Bonnie and Rene for your input...

The old trial sections have not only a lower dilution that didn't  
work, but a completely different antibody. the rest of the protocol  
would be the same.

it sounds as though if my old sections were understained it may be  
worth a shot.....but no guarantees :) if i'm understanding you  
correctly.

thanks!
CC

Cheryl Cross, DVM, Dipl. ACVP
Researcher
University Corporation for Atmospheric Research
College of Veterinary Medicine
University of Tennessee Department of Pathology
2407 River Drive, Room A201
Knoxville, TN 37996-4542
(423) 967-2724
fax: 865-974-5616
ccross <@t> ucar.edu





On Oct 16, 2007, at 4:58 PM, Rene J Buesa wrote:

> If the section already has DAB (as it probably does), you cannot  
> eliminate it.
> René J.
>
> Cheryl Cross <ccross6032 <@t> aol.com> wrote:
> hi everybody -
>
> I'm going back through some of my earlier attempts at immuno for
> cleaved caspase 3; i have a beautiful lesion i ruined :) on
> dilutions. is there anyway i can use my newer, better protocol on
> this previously stained slide?
>
> seems such a waste :)
>
> thanks for any input!
>
> CC
>
> Cheryl Cross, DVM, Dipl. ACVP
> Researcher
> University Corporation for Atmospheric Research
> College of Veterinary Medicine
> University of Tennessee Department of Pathology
> 2407 River Drive, Room A201
> Knoxville, TN 37996-4542
> (423) 967-2724
> fax: 865-974-5616
> ccross <@t> ucar.edu
>
>
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> Need a vacation? Get great deals to amazing places on Yahoo! Travel.