[Histonet] multiplex cytometry of mouse vaginal fluid for cytokine analysis

[Melissa Farmer]

I am desparately seeking information about whether anyone has successfully detected cytokines (TNFa, IFNy, IL-1, IL-4, IL-6) in mouse vaginal fluid using multiplex assays (specifically with the Luminex 100 system).  I fear that cytokine levels may not be detectable, and the literature using this technique is quite limited regardling rodent vaginal fluid.

Any wisdom?  A million thanks--

Melissa Farmer
Department of Psychology
McGill University