[Histonet] Iron staining

Thu Oct 11 12:48:24 CDT 2007

We have the Nexus special stainer and quit using the iron stain 2 years ago, as it did not give true staining on the iron for our bone marrows either.  Since we went back to doing them by hand, which is very easy, things are great in the world of FE staining!  We do all of our irons by hand as long as we have to have the reagents for the bone marrows and it works well for us!

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Sent: Thursday, October 11, 2007 12:13 PM
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Subject: Histonet Digest, Vol 47, Issue 13

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Today's Topics:

   1. RE: Iron Staining of Bone Marrows (Cheri Miller)
   2. Re: which mounting media for DAB + toluidine blue staining?
      (John Kiernan)
   3. renal immunofluorescence (Angela Bitting)
   4. FISH staining (Shtilkind, Dmitriy)
   5. FISH test (Shtilkind, Dmitriy)
   6. Re: which mounting media for DAB + toluidine blue staining?
      (Rene J Buesa)
   7. Re: FISH test (Rene J Buesa)

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Message: 1
Date: Thu, 11 Oct 2007 09:04:56 -0500
From: "Cheri Miller" <cmiller <@t> physlab.com>
Subject: RE: [Histonet] Iron Staining of Bone Marrows
To: "'Cheryl R. Kerry'" <tkngflght <@t> yahoo.com>,	"'Histonet'"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <005001c80c0f$b5edea50$3402a8c0 <@t> plab.local>
Content-Type: text/plain;	charset="us-ascii"

Well said! We gave up and did them by hand. They always work well and its so easy to do by hand

Cheri Miller HT ASCP
Histology Supervisor
Physicians Laboratory Services, Inc.
Omaha, NE 68117
402 738 5052

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheryl R. Kerry
Sent: Wednesday, October 10, 2007 11:27 AM
To: 'Histonet'
Subject: RE: [Histonet] Iron Staining of Bone Marrows

Hey Schaundra-

Have you talked with Ventana tech support?  They've probably dealt with similar issues from other clients and would know right off the bat how to help if there is help to be had.  If there isn't there always the old-fashioned alternative to fix this for your Path...

I know this isn't what you want to hear, but sometimes automation cannot duplicate what we can produce by hand.  If you try what they suggest and he's not getting what he needs to adequately diagnose the patient--he needs to serve the patient's best interest (an accurate diagnosis).  You may need to pull these off the machine.

Running them by hand isn't all that hard OR time-consuming if your reagents are at hand, and you do end up with a better product.  

My two cents having been in your shoes in the past...

Cheryl

Cheryl R. Kerry, HT(ASCP)
Full Staff Inc.
Staffing the AP Lab, one great tech at a time.
281.852.9457 office
281.883.7704 cell
800.756.3309 fax and alternate phone

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Schaundra Walton
Sent: Wednesday, October 10, 2007 11:04 AM
To: Histonet
Subject: [Histonet] Iron Staining of Bone Marrows

We use a Vetana NEXUS special stainer and stain our bone marrow aspirates and smears with Ventana's iron kit.  One of our pathologists has complained
(repeatedly) that our irons aren't staining like he expects.  Has anyone else had trouble with Ventana's iron kit?  Is anyone using a different protocol?  Any advise would be greatly appreciated.

Schaundra Walton BS HTL(ASCP)
Swedish American Hospital
1401 E. State St. 
Rockford, IL 61104

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Message: 2
Date: Thu, 11 Oct 2007 10:38:16 -0400
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] which mounting media for DAB + toluidine blue
	staining?
To: Moran Elishmereni <moran.elish <@t> gmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <db06e12659533.470dfd18 <@t> uwo.ca>
Content-Type: text/plain; charset=us-ascii

Yes. Use Entellan or any other resinous mounting medium. Nothing removes the oxidation product of DAB. 

John Kiernan
Anatomy, UWO
London, Canada
---
----- Original Message -----
From: Moran Elishmereni <moran.elish <@t> gmail.com>
Date: Thursday, October 11, 2007 8:16
Subject: [Histonet] which mounting media for DAB + toluidine blue staining?
To: histonet <@t> lists.utsouthwestern.edu

> Hi histonetters,
> 
> I am staining skin sections for mast cells (toluidine blue) and
> for an
> eosinophil protein (using DAB). Can I use entelan as mounting 
> media? It
> works nicely for the tol blue, but I fear it will ruin the DAB 
> staining. Is
> there any alternative?
> 
> Thanks,
> Moran
> 
> --
> Moran Elishmereni
> 
> Department of Pharmacology and Experimental Therapeutics School of 
> Pharmacy, Faculty of Medicine The Hebrew University of Jerusalem
> POB 12065
> Jerusalem 91120, ISRAEL
> Tel: 972-2-675-8746
> Fax: 972-2-675-8144
> Email: moran.elish <@t> gmail.com
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

------------------------------

Message: 3
Date: Thu, 11 Oct 2007 10:42:52 -0400
From: "Angela Bitting" <akbitting <@t> geisinger.edu>
Subject: [Histonet] renal immunofluorescence
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <470DFE2C020000C900010287 <@t> GHSGWIANW5V.GEISINGER.EDU>
Content-Type: text/plain; charset=US-ASCII

Where can I buy FITC-conjugated Lambda for direct immunofluorescent staining of renal bxs? Can someone recommend a reliable company?

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916

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Message: 4
Date: Thu, 11 Oct 2007 08:18:31 -0700
From: "Shtilkind, Dmitriy" <dshtilki <@t> uci.edu>
Subject: [Histonet] FISH staining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <2937D5939ABCCA4F96C14BBF3E463A1C03859D1B <@t> GUS.hs.uci.edu>
Content-Type: text/plain;	charset="us-ascii"

We are considering doing FISH in house. I would like to get some
opinions about equipment and all problems that go with that. Dako vs
Ventana etc...

Dmitriy Shtilkind, HTL (ASCP)
Histology Supervisor
UCI Medical Center
Orange, CA, 92868
(714) 456-8731
dshtilki <@t> uci.edu

------------------------------

Message: 5
Date: Thu, 11 Oct 2007 08:51:14 -0700
From: "Shtilkind, Dmitriy" <dshtilki <@t> uci.edu>
Subject: [Histonet] FISH test
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <2937D5939ABCCA4F96C14BBF3E463A1C03859D4C <@t> GUS.hs.uci.edu>
Content-Type: text/plain;	charset="us-ascii"

We are considering doing FISH in house. I would like to get some
opinions about equipment and all problems that go with that. Dako vs
Ventana etc...

Dmitriy Shtilkind, HTL (ASCP)
Histology Supervisor
UCI Medical Center
Orange, CA, 92868
(714) 456-8731
dshtilki <@t> uci.edu

------------------------------

Message: 6
Date: Thu, 11 Oct 2007 08:53:48 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] which mounting media for DAB + toluidine blue
	staining?
To: Moran Elishmereni <moran.elish <@t> gmail.com>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <107182.50125.qm <@t> web61219.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Moran:
Once the DAB has reacted (oxidized) it is permanent, no matter which mounting medium you use.
René J.
Moran Elishmereni <moran.elish <@t> gmail.com> wrote:  Hi histonetters,

I am staining skin sections for mast cells (toluidine blue) and for an
eosinophil protein (using DAB). Can I use entelan as mounting media? It
works nicely for the tol blue, but I fear it will ruin the DAB staining. Is
there any alternative?

Thanks,
Moran

-- 
Moran Elishmereni

Department of Pharmacology and Experimental Therapeutics
School of Pharmacy, Faculty of Medicine
The Hebrew University of Jerusalem
POB 12065
Jerusalem 91120, ISRAEL
Tel: 972-2-675-8746
Fax: 972-2-675-8144
Email: moran.elish <@t> gmail.com
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Message: 7
Date: Thu, 11 Oct 2007 08:55:12 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] FISH test
To: "Shtilkind, Dmitriy" <dshtilki <@t> uci.edu>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID: <178655.85336.qm <@t> web61221.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Try to contact Abbot to see their products.
  René J.

"Shtilkind, Dmitriy" <dshtilki <@t> uci.edu> wrote:

We are considering doing FISH in house. I would like to get some
opinions about equipment and all problems that go with that. Dako vs
Ventana etc...

Dmitriy Shtilkind, HTL (ASCP)
Histology Supervisor
UCI Medical Center
Orange, CA, 92868
(714) 456-8731
dshtilki <@t> uci.edu

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