FW: [Histonet] renal immunofluorescence

[Sebree Linda A.]

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: Normington Lacy 
Sent: Thursday, October 11, 2007 11:26 AM
To: Sebree Linda A.
Subject: RE: [Histonet] renal immunofluorescence


I use DakoCytomation.  The address is 6392 Via Real, Carpinteria, CA
93013.  The reference number is F0199.  I dilute the antibody using
their Background Reducing Diluent, reference number S3022.  It is
important to remember that when using in conjunction with Kappa, that
the protein ratio is taken into account for dilution purposes.  If my
procedure is needed, let me know.

Lacy

-----Original Message-----
From: Sebree Linda A. 
Sent: Thursday, October 11, 2007 10:29 AM
To: Normington Lacy
Subject: FW: [Histonet] renal immunofluorescence


Lacy,

Is this something you can help with?

Linda Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Angela
Bitting
Sent: Thursday, October 11, 2007 9:43 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] renal immunofluorescence


Where can I buy FITC-conjugated Lambda for direct immunofluorescent
staining of renal bxs? Can someone recommend a reliable company?

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916



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