[Histonet] which mounting media for DAB + toluidine blue staining?

[John Kiernan]

Yes. Use Entellan or any other resinous mounting medium. Nothing removes the oxidation product of DAB. 

John Kiernan
Anatomy, UWO
London, Canada
---
----- Original Message -----
From: Moran Elishmereni <moran.elish <@t> gmail.com>
Date: Thursday, October 11, 2007 8:16
Subject: [Histonet] which mounting media for DAB + toluidine blue staining?
To: histonet <@t> lists.utsouthwestern.edu

> Hi histonetters,
> 
> I am staining skin sections for mast cells (toluidine blue) and 
> for an
> eosinophil protein (using DAB). Can I use entelan as mounting 
> media? It
> works nicely for the tol blue, but I fear it will ruin the DAB 
> staining. Is
> there any alternative?
> 
> Thanks,
> Moran
> 
> -- 
> Moran Elishmereni
> 
> Department of Pharmacology and Experimental Therapeutics
> School of Pharmacy, Faculty of Medicine
> The Hebrew University of Jerusalem
> POB 12065
> Jerusalem 91120, ISRAEL
> Tel: 972-2-675-8746
> Fax: 972-2-675-8144
> Email: moran.elish <@t> gmail.com
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