[Histonet] stain living cells - what dye doesn't kill the cells?

[Philip Oshel]

Try 0.01% aqueous Neutral Red.

Phil

>Dear Histonetters,
>
>Does anyone know of a dye/stain (fluorescent or non-fluorescent) that does
>not kill cells? I want to do some live-imaging of live primary mast cells,
>but be able to distinguish them from other cells in a certain mix. So I need
>to stain them- but not affect there viability/function.
>I know that this isnt exactly the forum for this question, but I'm hoping to
>get some suggestions anyway.
>Many thanks,
>Moran
>
>--
>Moran Elishmereni
>
>Department of Pharmacology and Experimental Therapeutics
>School of Pharmacy, Faculty of Medicine
>The Hebrew University of Jerusalem
>POB 12065
>Jerusalem 91120, ISRAEL
>Tel: 972-2-675-8746
>Fax: 972-2-675-8144
>Email: moran.elish <@t> gmail.com
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-- 
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859