[Histonet] Sucrose and paraffin

[Molinari, Betsy]

Shawn,
 I have heard of sucrose for cryoprotection, but these are tissues that
have been fixed in 10% NBF and they want to then put in sucrose then
process for paraffin. I just don't understand the rational or if there
would be any advantage for IHC.
Betsy

-----Original Message-----
From: Shawn Leslie [mailto:LeslieS <@t> vetmed.ufl.edu] 
Sent: Thursday, October 04, 2007 6:57 AM
To: Molinari, Betsy
Subject: Re: [Histonet] Sucrose and paraffin

I used sucrose in research for cryo protection. We used 10, 20. and 30%
sucrose to protect the tissue from freeze artifact as we were doing
frozen sections on it. I don't think that sucrose will do anything for
antigen staining....



Shawn Leslie
Scientific Research Manager
Anatomic Pathology
University of Florida
School of Veterinary Medicine
352-392-2235 ext 4555


>>> "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu> 10/4/2007 6:48 AM
>>>
Hi all,

 I have no experience with IHC but (once again) had a researcher ask me
if placing tissue in sucrose after 10% NBF is helpful in antigen
staining.

Thanks,

Betsy

 

Betsy Molinari HT (ASCP)

Texas Heart Institute

Cardiovascular Pathology

6770 Bertner Ave.

Houston,TX 77030

832-355-6524

832-355-6812 (fax)

 

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