[Histonet] Melan-A on Dako Immuno Stainer

[Miley, Joannah C.]

Our lab is demoing a Dako immuonstainer and I am having a hard time
optimizing our Melan-A.  The dilutions I have tried are 1:50, 1:100, and
1:200.  I have tried antigen retreival with EDTA pH9 and Citrate buffer
at pH 6.  I am incubating in the primary and polymer for 20 minutes
each.  I am still having a lot of trouble with excessive muddy
background.  Any ideas??
 
Thanks,
 
Joannah Miley, HT(ASCP)
NW Pathology
Bellingham, WA