[Histonet] benzidine stain to demonstrate red blood cells in whole embryos

[Derek Papalegis]

Rogers, Rhonda wrote:
> Good morning,
>
> I am looking for a method to stain whole mouse embryos with benzidine to demonstrate red blood cells.  The one we were given by a colleague is not working for us, so I hope someone on Histonet is willing to share a reliable method.
>
> Regards,
>
> Rhonda
>
>
> Rhonda Rogers, HTL(ASCP)
>
> Wells Center for Pediatric Research - Conway Laboratory
>
> Indiana University Purdue University Indianapolis
>
> 1044 W. Walnut St., R4 379
>
> Indianapolis, IN  46202-5225
>
>
>
> Phone: 317-278-8781     E-mail: rhrogers <@t> iupui.edu
>
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Hi Rhonda,
I recently had an investigator request this. We got the best results by 
staining the mouse embryos with Benzadine after fixation in Bouin's. I 
believe we stained them for 30 minutes in Benzadine. After staining, 
just process and cut like normal and you should see staining on the 
tissue. In addition to the Benzadine staining, we stained the slides 
with Okajima's and the investigator was very happy with the results.

I hope this helps,
Derek

-- 
Derek Papalegis HT (ASCP)
Histotechnician
Division of Laboratory Animal Medicine
Tufts University 
136 Harrison Avenue
Boston, MA 02111
phone: 617 636-2971
fax: 617 636-8354