[Histonet] NaCl in antibody diluent

[Anthony Reilly]

Hi 

NaCl is a normal component of PBS.  The high salt buffer DAKO are most
likely referring to is TBS which is a Tris buffer with 0.9% NaCl added. 
As others have stated this is used to reduce background.  In my
experience this will only give minimal improvement.  If you are
suffering background you are best to investigate your dilution, blocking
if any and the type of detection system used.

regards


Tony Reilly
Chief Scientist
Anatomical Pathology
Pathology Queensland
Prince Charles Hospital
Rode Rd Chermside Q 4032
Australia
Ph: 07 3139 4543
Fax: 07 3193 4546
tony_reilly <@t> health.qld.gov.au


>>> "Harvey, Jennifer Lynn" <jennifer.harvey <@t> Vanderbilt.Edu> 11/28/07
3:28 am >>>
Histonet,

 

Has anyone tried NaCl in there diluent buffer for IHC, if so what
concentration. I read about it in DAKO's immunostaining methods
booklet
but they don't state the concentration. This is my last chance with my
background problem. I have a sticky collagen issue I cant get rid of.

 

 

 

Thanks!

 

 

Jennifer Harvey, HT(ASCP) QIHC

Vanderbilt Vision Research Center

RM 8105 MCE North Tower

1215 21st Ave. South

Nashville, TN  37232-8808

Phone: 615-936-1486

 

 

 

 

 

 

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