[Histonet] NaCl in antibody diluent

Tue Nov 27 11:28:27 CST 2007

Histonet,

Has anyone tried NaCl in there diluent buffer for IHC, if so what
concentration. I read about it in DAKO's immunostaining methods booklet
but they don't state the concentration. This is my last chance with my
background problem. I have a sticky collagen issue I cant get rid of.

Thanks!

Jennifer Harvey, HT(ASCP) QIHC

Vanderbilt Vision Research Center

RM 8105 MCE North Tower

1215 21st Ave. South

Nashville, TN  37232-8808

Phone: 615-936-1486