[Histonet] SelecTech Staining

Greg Dobbin gvdobbin <@t> ihis.org
Tue Nov 27 11:03:40 CST 2007

I am using them on the Dako Autostainer XL and sure I'll share!
Here is our schedule:

Oven, 65C 10:00
Xylene 3 min
Xylene 2 min
Abs. ETOH 1 min
Abs. ETOH 1 min
95% ETOH 1 min
70% ETOH 1 min
Wash, Tap H2O 2 min
Wash, dist. H2O 1 min
Hematox. 560 4 min
Wash, Tap H2O 2 min
Define 1 min
Wash, Tap H2O 2 min
Bluing 1 min
Wash, Tap H2O
70% ETOH 1 min
Alc. Eosin 515 30 sec
Abs. ETOH 1 min
Abs. ETOH 1 min
Abs. ETOH 1 min
Xylene 2 min
Xylene 2 min

We (the technologists) love the these reagents-no more filtering, and
the docs love the staining. We have toyed with the idea of adding a 50%
ETOH step after eosin to reduce the eosin intensity, but we would have
to lose another station and the docs are happy with it as it is, sooo
for now we will not be bothered. Good luck with them.

Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!

>>> "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu> 11/27/2007 12:00 PM >>>
Is anyone out there using Surgipath's SelecTech stains (Blue Buffer
Define) with a regressive hematoxylin on a LEICA AUTOSTAINER XL?  If
would you be willing to offer your staining protocol (setup and
Thanks so much!


Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106



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