AW: [Histonet] frozens from lung-tissue

[Gudrun Lang]

Kemlo,
My original question was, how to get the best frozen lung-slides without the
use of liquid nitrogen or isopentan. - just with the cryocut, chucks and
OCT.
The pathologist complains about "overfrozen" edges and a bad overall
morphology. I will ask him for more descriptionary details or get a picture.
Thank you for your hints.

Gudrun Lang
 
Biomed. Analytikerin
Histolabor
Akh Linz
Krankenhausstr. 9
4020 Linz
+43(0)732/7806-6754
-----Ursprüngliche Nachricht-----
Von: Kemlo Rogerson [mailto:Kemlo.Rogerson <@t> waht.swest.nhs.uk] 
Gesendet: Donnerstag, 22. November 2007 10:42
An: gu.lang <@t> gmx.at; histonet <@t> lists.utsouthwestern.edu
Betreff: RE: [Histonet] frozens from lung-tissue

You can't practically get any colder than liquid nitrogen and if you
don't want ice artefact then I guess you have to use it. The only issue
sometimes is that you can get a bubble of nitrogen around the tissue
that acts as an insulator; maybe you can freeze in/ on metal foil?

What are the problems you have or why is your boss unhappy? If it's ice
crystal artefact you must freeze faster, if it's nuclear staining, try
post fixing the slides before staining in an alcohol. 

Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
The road to success is dotted with many tempting parking places.
--Author Unknown 

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