AW: [Histonet] frozens from lung-tissue

Gudrun Lang gu.lang <@t>
Fri Nov 23 09:14:45 CST 2007

My original question was, how to get the best frozen lung-slides without the
use of liquid nitrogen or isopentan. - just with the cryocut, chucks and
The pathologist complains about "overfrozen" edges and a bad overall
morphology. I will ask him for more descriptionary details or get a picture.
Thank you for your hints.

Gudrun Lang
Biomed. Analytikerin
Akh Linz
Krankenhausstr. 9
4020 Linz
-----Ursprüngliche Nachricht-----
Von: Kemlo Rogerson [mailto:Kemlo.Rogerson <@t>] 
Gesendet: Donnerstag, 22. November 2007 10:42
An: gu.lang <@t>; histonet <@t>
Betreff: RE: [Histonet] frozens from lung-tissue

You can't practically get any colder than liquid nitrogen and if you
don't want ice artefact then I guess you have to use it. The only issue
sometimes is that you can get a bubble of nitrogen around the tissue
that acts as an insulator; maybe you can freeze in/ on metal foil?

What are the problems you have or why is your boss unhappy? If it's ice
crystal artefact you must freeze faster, if it's nuclear staining, try
post fixing the slides before staining in an alcohol. 

Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
The road to success is dotted with many tempting parking places.
--Author Unknown 

This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful.
Please inform me that this message has gone astray before deleting it.
Thank you for your co-operation

More information about the Histonet mailing list