[Histonet] Frozens from Lung tissue

PETER CRAVEN peter.craven <@t> haht.scot.nhs.uk
Thu Nov 22 08:14:48 CST 2007


We used to embed our lung blocks in Gelatin prior to freezing in OCT to
help hold the lung together when frozen sectioned

Rinse out the formalin

10% Gelatin till the block of tissue sinks

20% Gelatin overnight

Change to fresh 20% and set in a fridge to set the gelatin

Trim off the excess gelatin

Adhere to cryostat block with OCT and cut

Hope this helps




Peter L Craven FIBMS


Pathology Department

Raigmore Hospital

Inverness IV2 3UJ

tel. 01463 704000 ext. 4269

email: peter.craven <@t> haht.scot.nhs.uk 


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