[Histonet] Frozens from Lung tissue

[PETER CRAVEN]

Gudrun 

We used to embed our lung blocks in Gelatin prior to freezing in OCT to
help hold the lung together when frozen sectioned

Rinse out the formalin

10% Gelatin till the block of tissue sinks

20% Gelatin overnight

Change to fresh 20% and set in a fridge to set the gelatin

Trim off the excess gelatin

Adhere to cryostat block with OCT and cut

Hope this helps

Peter

 

 

Peter L Craven FIBMS

BMS 2

Pathology Department

Raigmore Hospital

Inverness IV2 3UJ

tel. 01463 704000 ext. 4269

email: peter.craven <@t> haht.scot.nhs.uk 

 


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