[Histonet] frozens from lung-tissue

[Gudrun Lang]

Hi to all,

I need the help of those, who have experience on making good frozens from
lung-tissue. We look for a method for freezing lung-tissue without the usage
of liquid nitrogen, co2 or isopentan. - Just on the cryocut. The head of the
institute is not satisfied with the quality and wants us to do better. Our
usual method is to make a OCT-layer on a chuck, let it freeze, give the
tissue on it, surround with OCT, put the cold weight on it, let it freeze
and make the sections.

We tried putting the tissue in the liquid OCT on the chuck without a layer
and  freezing it in a mold with the chuck on top. I also tried first
freezing the piece solely in liquid nitrogen or isopentan, then putting it
on a chuck. All in all - my boss wasn't happy with it.

 

Any input will be appreciated. Thank you in advance

Gudrun Lang