[Histonet] Minimum time processing

Tony Henwood AnthonyH <@t> chw.edu.au
Sun Nov 18 16:18:46 CST 2007

Most important to ensure adequate fixation - as long as you can. At a
minimum we fix 2-3mm diameter core bxs in 10% NBF for
 one hour and then 5 changes of alcohol 5 min each, xylene (x3) 5 min
each and wax (x3) 5min each.

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Webb,
Dorothy L
Sent: Saturday, 17 November 2007 6:10 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Minimum time processing

For those histology labs doing prostate needle biopsies.....I need some
advice!!  We process our pnbx on a separate program on a VIP 5 and these
biospies are taken with a 20 gauge needle, very thin.  What would be the
minimum times you would recommend processing in the reagents?  I am
currently doing 10 minutes per station and I am seeing some very dry
cores (they are usually the tiniest ones) that, of course, are staining
very dark.  I have a pathologist that  feels we can do better, so
needing my super histotechs to help me out once again!!!  Thank you So
much for  help you always send via the histonet!!

Dorothy Webb, HT (ASCP)
Histology Technical Supervisor 
Regions Hospital, Pathology Department 
640 Jackson Street, Saint Paul, MN 55101-2595 
Phone: 651-254-2962
Fax: 651-254-2741 
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