mpence <@t> grhs.net
Thu Nov 15 16:43:56 CST 2007
What detection kit are you using?
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Sent: Thursday, November 15, 2007 4:40 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] MyoD1
I noticed a few other Histonetters with problems with their MyoD1.
Here's my story. I use the Dako Cytomation concentrate-clone 5.8A. ON
the Ventana Benchmark, my rpotocol is CC1-Mild, 60 minute incubation at
42 degrees (increased heat). I was using the antibody at a 1:100
dilution but when I started seeing poor/weak staining, I tried 1:50 and
1:20. My results were basically the same from one dilution to the other.
There was varying degrees of background staining in both Rhabdo and
skeletal muscle tissue and very rare cytoplasmic staining in the
Rhabdo's. This is supposed to be a nuclear stain. I'm not sure if I have
ever had good, consistent nuclear staining at any dilution.
Any thoughts, comments, etc?........................
Chris Jacobs, IHC Specialist
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