[Histonet] MyoD1

Christine Jacobs jacobc <@t> mmc.org
Thu Nov 15 16:39:58 CST 2007

I noticed a few other Histonetters with problems with their MyoD1. 
Here's my story. I use the Dako Cytomation concentrate-clone 5.8A. ON the Ventana Benchmark, my rpotocol is CC1-Mild, 60 minute incubation at 42 degrees (increased heat).
I was using the antibody at a 1:100 dilution but when I started seeing poor/weak staining, I tried 1:50 and 1:20. My results were basically the same from one dilution to the other. There was varying degrees of background staining in both Rhabdo and skeletal muscle tissue and very rare cytoplasmic staining in the Rhabdo's. This is supposed to be a nuclear stain. I'm not sure if I have ever had good, consistent nuclear staining at any dilution. 
Any thoughts, comments, etc?........................
Chris Jacobs, IHC Specialist
NorDx Labs
Scarborough, ME 

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