[Histonet] glycosaminoglycan (GAG).

[Patsy Ruegg]

Thanks Geoff,
Do you think formic acid decal will have any adverse effect on the ffpe
samples I want to do this on?
Patsy

-----Original Message-----
From: Geoff McAuliffe [mailto:mcauliff <@t> umdnj.edu] 
Sent: Wednesday, November 14, 2007 12:31 PM
To: Patsy Ruegg
Cc: 'Histonet'
Subject: Re: [Histonet] glycosaminoglycan (GAG).

Hi Patsy:

    Alcian Blue (1% AB in 3% acetic acid) at pH 2.5 will stain both 
carboxylated and sulfated GAG. At pH 1.0 only sulfated GAG will stain.

Geoff

Patsy Ruegg wrote:
> Does anyone know of a specific marker for this, antibody or special stain?
> I know we have used saffrinin o, azure b at different ph's and maybe some
> collagen antibodies in my past life working in bone, but maybe there is
> something else out there I have missed?
>
>  
>
> Patsy
>
>  
>
>  
>
> Patsy Ruegg, HT(ASCP)QIHC
>
> IHCtech, LLC
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
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