[Histonet] glycosaminoglycan (GAG).

[Geoff McAuliffe]

Hi Patsy:

    Alcian Blue (1% AB in 3% acetic acid) at pH 2.5 will stain both 
carboxylated and sulfated GAG. At pH 1.0 only sulfated GAG will stain.

Geoff

Patsy Ruegg wrote:
> Does anyone know of a specific marker for this, antibody or special stain?
> I know we have used saffrinin o, azure b at different ph's and maybe some
> collagen antibodies in my past life working in bone, but maybe there is
> something else out there I have missed?
>
>  
>
> Patsy
>
>  
>
>  
>
> Patsy Ruegg, HT(ASCP)QIHC
>
> IHCtech, LLC
>
> 12635 Montview Blvd. Ste.215
>
> Aurora, Colorado 80045
>
> Phone: 720-859-4060
>
> Fax: 720-859-4110
>
> pruegg <@t> ihctech.net
>
> www.ihctech.net 
>
> www.ihcrg.org 
>
>  
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
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