[Histonet] processing

Marshall Terry Dr, Consultant Histopathologist Terry.Marshall <@t> rothgen.nhs.uk
Wed Nov 14 11:53:34 CST 2007

Maxim, it's hard to credit that slides are still processed manually. How
many in a day?


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Maxim
Sent: 14 November 2007 17:47
To: Dorothy.L.Webb <@t> HealthPartners.Com
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] processing

We always fixes our breast specimens with 10% formalin and earlier have
had great troubles in processing and cutting. When we started use
acetone as dehydratant and mixtures of aliphatic hydrocarbons
(C7-C10) as clearant, then we got much better results than earlier, but
some difficultes in cut still here. Now we use isopropanol as
dehydratant and mineral oil as clearant and have best results without
any problems. We process all our tissues manually and simultaneously
with all specimens, including curretage, bone, uterus, skin etc. Our
pathologists and pathologists from other hospitals like these slides.
Next year we will be do IHC for breast.
Maxim Peshkov

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