[Histonet] TMA arrayers. Does anyone have a favorite model ?
Bernice Frederick
b-frederick <@t> northwestern.edu
Thu Nov 8 08:17:25 CST 2007
To all,
Did anyone see the TMA template (Quick-Ray from Sakura)? It seemed to be
kind of neat as well as simple, especially for those 1mm cores. There is a
demo video on the website.
Bernice
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pierre
CHAUMAT
Sent: Wednesday, November 07, 2007 5:42 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] TMA arrayers. Does anyone have a favorite model ?
Dear all,
Greg, you mentionned Boekeler but on their web site, I could not find any
tissuer arrayer. Would you have a link to an exact page ?
Thanks
Pierre
-----Message d'origine-----
De : histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] De la part de
histonet-request <@t> lists.utsouthwestern.edu
Envoyé : mardi 6 novembre 2007 16:04
À : histonet <@t> lists.utsouthwestern.edu
Objet : Histonet Digest, Vol 48, Issue 6
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Today's Topics:
1. TMA arrayers. Does anyone have a favorite model? (Hugh Luk)
2. Deplasticizing MMA sections (Nancy W. Troiano)
3. Histo, cyto job in NY (Xorren <@t> aol.com)
4. Re: TMA arrayers. Does anyone have a favorite model?
(Greg Dobbin)
5. NSH Report (Breeden, Sara)
6. Re: TMA arrayers. Does anyone have a favorite model?
(Thomas Pier)
7. Re: Histonet Digest, Vol 48, Issue 5 (Boneslides <@t> aol.com)
8. RE: deplasticizing PMMA sections for staining (Monfils, Paul)
9. Re: TMA arrayers. Web link (Greg Dobbin)
10. NSH show (Rathborne, Toni)
11. RE: Mucin overstained with Hematoxylin in G.I. specimens
(Charles.Embrey)
12. RE: Mucin overstained with Hematoxylin in G.I. specimens
(Bonnie Whitaker)
13. RE: Mucin overstained with Hematoxylin in G.I. specimens
(Douglas D Deltour)
14. RE: deplasticizing PMMA sections for staining
(Yaskovich, Ruth A (NIH/NIDCR) [E])
15. NSH (Bernice Frederick)
16. RE: Mucin overstained with Hematoxylin in G.I. specimens
(LuAnn Anderson)
17. how to keep thick sections on the slides after deparaffin
(FU,DONTAO)
18. Re: Mucin overstained with Hematoxylin in G.I. specimens (DNon)
19. RE: how to keep thick sections on the slides after deparaffin
(Kemlo Rogerson)
20. FYI - Brainbow (Barbara Webb)
21. RE: Histo, cyto job in NY (Lee & Peggy Wenk)
22. RELIA's Histology Opportunities Update 11-06-07 (Pam Barker)
23. RE: Histo, cyto job in NY (James Watson)
----------------------------------------------------------------------
Message: 1
Date: Mon, 5 Nov 2007 08:22:56 -1000
From: Hugh Luk <hlukey <@t> msn.com>
Subject: [Histonet] TMA arrayers. Does anyone have a favorite model?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY116-W173686820D8D949EB8E3B5C3880 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
I need advice. Is there a favorite Tissue microarray unit? I currently use
the Beecher TMA #1, but due to the humidity in Hawaii, the electronics have
failed. I guess I shouldn't have taken it swimming with me in the ocean! I
attending NSH, but did not see too many TMA vendors. Beecher did throw a
very nice break-time pretzel party, but I missed meeting any of their
people. I would appreciate any advice... Thanks,
Hugh Luk, HTL (ASCP)
hlukey <@t> msn.com
or hluk <@t> crch.hawaii.edu
Pathology Shared Resources Lab
Cancer Research Center of Hawaii
Honolulu, Hawaii
_________________________________________________________________
Climb to the top of the charts! Play Star Shuffle: the word scramble
challenge with star power.
http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_oct
------------------------------
Message: 2
Date: Mon, 05 Nov 2007 13:25:52 -0500
From: "Nancy W. Troiano" <nancy.troiano <@t> yale.edu>
Subject: [Histonet] Deplasticizing MMA sections
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <5.2.1.1.2.20071105132352.00c3f668 <@t> email.med.yale.edu>
Content-Type: text/plain; format=flowed; charset=us-ascii
Try soaking the slides in two 5 minute changes of acetone (100%) to
deplastify or you can deplastify in 2 changes of Cellosolve (Fisher, cat.
#E181-4) 25 minutes each, followed by 5 minutes in 70% ethanol, 40%
ethanol, then distilled water for five minutes prior to doing your staining.
------------------------------
Message: 3
Date: Mon, 5 Nov 2007 13:44:13 EST
From: Xorren <@t> aol.com
Subject: [Histonet] Histo, cyto job in NY
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <cfd.1f547405.3460be7d <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Hello all !
Does anyone know of any histology or cytology prep job in the NY area.
I have have 15 years in the field and hope someone has some info.
you can respond @ xorren <@t> aol.com or call (516) 671-0907
have a great day !!!!!
**************************************
See what's new
at http://www.aol.com
------------------------------
Message: 4
Date: Mon, 05 Nov 2007 14:44:33 -0400
From: "Greg Dobbin" <gvdobbin <@t> ihis.org>
Subject: Re: [Histonet] TMA arrayers. Does anyone have a favorite
model?
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s72f2c58.087 <@t> ihis.org>
Content-Type: text/plain; charset=US-ASCII
I am investigating an instrument that I saw at NSH by RMC Products.
Model no. is KIN-1. The picture on the web site does not match exactly
the unit they had at NSH but they could send you the same broshure they
were passing out at their booth (it must be available as a pdf).
Cheers!
Greg
Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385
There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!
>>> hlukey <@t> msn.com 11/5/2007 2:22 PM >>>
I need advice. Is there a favorite Tissue microarray unit? I
currently use the Beecher TMA #1, but due to the humidity in Hawaii, the
electronics have failed. I guess I shouldn't have taken it swimming
with me in the ocean! I attending NSH, but did not see too many TMA
vendors. Beecher did throw a very nice break-time pretzel party, but I
missed meeting any of their people. I would appreciate any advice...
Thanks,
Hugh Luk, HTL (ASCP)
hlukey <@t> msn.com
or hluk <@t> crch.hawaii.edu
Pathology Shared Resources Lab
Cancer Research Center of Hawaii
Honolulu, Hawaii
_________________________________________________________________
Climb to the top of the charts! Play Star Shuffle: the word scramble
challenge with star power.
http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_oct__
_____________________________________________
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------------------------------
Message: 5
Date: Mon, 5 Nov 2007 11:50:55 -0700
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] NSH Report
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4D14F0FC9316DD41972D5F03C070908B8F4725 <@t> nmdamailsvr.nmda.ad.nmsu.edu>
Content-Type: text/plain; charset="us-ascii"
Okay, I'm back from my week at NSH and this is my report.
1. The NSH staff is awesome. Aubrey, Carrie and the entire NSH
staff pulled off yet another flawless meeting. We can't thank NSH
enough for all they do!
2. Joe Nocito is alive and well. I met him. He lives and he
wasn't wearing a flak jacket.
3. Our vendors are magnificent - supportive, creative, helpful and
approachable. Kudos!!
4. Each one of us needs to recruit NSH members - there is strength
in numbers. NSH is our connection in SO many ways!
5. Ada Feldman makes a terrific clown!
6. Did you know that histologists are the ONLY technicians not
recognized by the US Government as healthcare professionals?
7. After 3 days of walking the 6 blocks from hotel to convention
center, I discovered the free bus.
8. There must be a "message board" at next year's meeting so we can
find each other if we need to.
9. I want to know what vitamins Peggy Wenk takes. Seriously.
Sally Breeden, HT(ASCP)
NM Dept. of Agriculture
Veterinary Diagnostic Services
PO Box 4700
Albuquerque, NM 87106
505-841-2576
------------------------------
Message: 6
Date: Mon, 05 Nov 2007 12:55:36 -0600
From: "Thomas Pier" <tp2 <@t> medicine.wisc.edu>
Subject: Re: [Histonet] TMA arrayers. Does anyone have a favorite
model?
To: <histonet <@t> lists.utsouthwestern.edu>, <hlukey <@t> msn.com>
Message-ID: <472F12C8020000DF00002B3D <@t> gwmail.medicine.wisc.edu>
Content-Type: text/plain; charset=US-ASCII
I haven't used any other instruments, but I've been happy with the Beecher
MTA-1.
Tom Pier
>>> Hugh Luk <hlukey <@t> msn.com> 11/05/07 12:22 PM >>>
I need advice. Is there a favorite Tissue microarray unit? I currently use
the Beecher TMA #1, but due to the humidity in Hawaii, the electronics have
failed. I guess I shouldn't have taken it swimming with me in the ocean! I
attending NSH, but did not see too many TMA vendors. Beecher did throw a
very nice break-time pretzel party, but I missed meeting any of their
people. I would appreciate any advice... Thanks,
Hugh Luk, HTL (ASCP)
hlukey <@t> msn.com
or hluk <@t> crch.hawaii.edu
Pathology Shared Resources Lab
Cancer Research Center of Hawaii
Honolulu, Hawaii
_________________________________________________________________
Climb to the top of the charts! Play Star Shuffle: the word scramble
challenge with star power.
http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_oct__
_____________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Mon, 5 Nov 2007 14:10:40 EST
From: Boneslides <@t> aol.com
Subject: [Histonet] Re: Histonet Digest, Vol 48, Issue 5
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <bbf.1b6dfabb.3460c4b0 <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
I de-plasticize my PMMA sections in methyl acetate...takes about 30
minutes,
then rehydrate thru several changes of absolute, 95% and 70% alcohol, rinse
in water, stain as desired.
Diane M. Mahovlic, HT, MLT(ASCP)
Orthopedic Pathology & Biomaterials Laboratory
Department of Anatomic Pathology
The Cleveland Clinic Foundation
9500 Euclid Avenue- L30
Cleveland, Ohio 44195
216-444-0166
************************************** See what's new at http://www.aol.com
------------------------------
Message: 8
Date: Mon, 5 Nov 2007 14:22:08 -0500
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: RE: [Histonet] deplasticizing PMMA sections for staining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4EBFF65383B74D49995298C4976D1D5E273CF6 <@t> LSRIEXCH1.lsmaster.lifespan.org>
Content-Type: text/plain; charset="iso-8859-1"
In my experience, xylene is a rather poor solvent for PMMA. It works much
faster at 60 degrees, but don't try heating flammable solvents in your oven
unless you are certain it is certified explosion-proof (most paraffin ovens
are not). Even then, unless you have the oven in or at least next to a fume
hood, you'll get a lot of xylene vapors in the air as a result of heating
it. I deplasticise PMMA with 2-methoxyethyl acetate, which only takes a
couple of hours at room temperature. This has a rather strong though not
unpleasant odor, and should be used in the hood.
------------------------------
Message: 9
Date: Mon, 05 Nov 2007 16:12:32 -0400
From: "Greg Dobbin" <gvdobbin <@t> ihis.org>
Subject: Re: [Histonet] TMA arrayers. Web link
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <s72f4101.034 <@t> ihis.org>
Content-Type: text/plain; charset=US-ASCII
Sorry. I see the company name is actually Boeckeler and the website is
www.boeckeler.com
Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385
There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!
>>> "Greg Dobbin" <gvdobbin <@t> ihis.org> 11/5/2007 2:44 PM >>>
I am investigating an instrument that I saw at NSH by RMC Products.
Model no. is KIN-1. The picture on the web site does not match exactly
the unit they had at NSH but they could send you the same broshure
they
were passing out at their booth (it must be available as a pdf).
Cheers!
Greg
Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385
There is some merit in doing the right thing rather badly,
but absolutely none in doing the wrong thing excellently!
>>> hlukey <@t> msn.com 11/5/2007 2:22 PM >>>
I need advice. Is there a favorite Tissue microarray unit? I
currently use the Beecher TMA #1, but due to the humidity in Hawaii,
the
electronics have failed. I guess I shouldn't have taken it swimming
with me in the ocean! I attending NSH, but did not see too many TMA
vendors. Beecher did throw a very nice break-time pretzel party, but I
missed meeting any of their people. I would appreciate any advice...
Thanks,
Hugh Luk, HTL (ASCP)
hlukey <@t> msn.com
or hluk <@t> crch.hawaii.edu
Pathology Shared Resources Lab
Cancer Research Center of Hawaii
Honolulu, Hawaii
_________________________________________________________________
Climb to the top of the charts! Play Star Shuffle: the word scramble
challenge with star power.
http://club.live.com/star_shuffle.aspx?icid=starshuffle_wlmailtextlink_oct__
_____________________________________________
Histonet mailing list
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de votre syst?me informatique.
------------------------------
Message: 10
Date: Mon, 5 Nov 2007 15:30:49 -0500
From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
Subject: [Histonet] NSH show
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E78340C766A5284D999F5F5891DDF89007D16BCD <@t> smcmail.somerset-healthcare.com>
Content-Type: text/plain; charset="utf-8"
How was the show? I heard there were a lot of vendors there. What about the
lectures/workshops? I was hoping to go, so many relevant topics offered. A
friend of mine was there and mentioned that Ventana had some sort of
secretive booth. What was that all about? Is it a new IHC stainer? Or part
of their whole "lab platform"?
Toni
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Message: 11
Date: Mon, 5 Nov 2007 15:08:54 -0600
From: "Charles.Embrey" <Charles.Embrey <@t> carle.com>
Subject: RE: [Histonet] Mucin overstained with Hematoxylin in G.I.
specimens
To: "DNon" <d.non <@t> verizon.net>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<44780C571F28624DBB446DE55C4D733A1FE565 <@t> EXCHANGEBE1.carle.com>
Content-Type: text/plain; charset="us-ascii"
I find this post most interesting. After using Surgipath Gills III for
the past 7 years we started having a problem with blue mucin just last
month. I corrected the problem by adding GAA but I now have to monitor
the pH on a daily basis. It is strange that after all these years I
would suddenly have this problem and then read about another lab having
the same difficulty with Surgipath Hematoxylin starting about the same
timeframe. Has Surgipath changed something?
Charles Embrey Jr., PA(ASCP)
Histology Manager, Carle Clinic
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of DNon
Sent: Saturday, November 03, 2007 9:42 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mucin overstained with Hematoxylin in G.I. specimens
Hello Histonet,
Over the past several weeks our lab has had problems with
hematoxylin overstaining in the mucin of our gastric specimens. The
degree of hematoxylin overstaining is alarming in the mucin of the
gastric specimens but absent or negligible in other specimens. One
pathologist reviewing the slides indicated some of the overstained
specimens appeared to be inadequately fixed. We have addressed that
issue which showed some improvement, but the problem persists. We are
making changes to increase fixation time (although the specimens now
appear adequately fixed yet retain a large measure of the overstaining),
but any further suggestions for corrective action would be much
appreciated.
We've been hesitant to increase acid alcohol clearing time because
our other specimens are staining well with the current protocol.
We are using the following reagents and stain times:
- Surgipath specimen containers pre-filled with 10% neutral buffered
Formalin.
- Surgipath Gills III Hematoxlyin - 6.5 minutes followed by running
water washes
- .75% HCL in 70% ETOH for 1 second (1 dip) followed by running water
wash
- Surgipath Scott's Tap Water for 1 min followed by running water wash
Sakura automated stainer set to agitate ( up and down dipping of racks )
once every 2 seconds, which is the fastest speed available on it.
Dick Non
Pathology Department
Ocean County Medical Lab
Brick, New Jersey
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Mon, 5 Nov 2007 15:22:45 -0600
From: "Bonnie Whitaker" <bwhitaker <@t> brownpathology.com>
Subject: RE: [Histonet] Mucin overstained with Hematoxylin in G.I.
specimens
To: "'Charles.Embrey'" <Charles.Embrey <@t> carle.com>, "'DNon'"
<d.non <@t> verizon.net>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <003501c81ff2$02602060$3601a8c0 <@t> brownpathology.net>
Content-Type: text/plain; charset="us-ascii"
Hi All,
I had the same type incident (with the increase in mucin staining with the
Surgipath hematoxylin), but it was a one time thing that we attributed to a
larger than normal volume of H&E's in that particular time period. We
increased the frequency that solutions are changed slightly, and haven't had
another problem.
Bonnie Whitaker
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Charles.Embrey
Sent: Monday, November 05, 2007 3:09 PM
To: DNon; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Mucin overstained with Hematoxylin in G.I. specimens
I find this post most interesting. After using Surgipath Gills III for the
past 7 years we started having a problem with blue mucin just last month. I
corrected the problem by adding GAA but I now have to monitor the pH on a
daily basis. It is strange that after all these years I would suddenly have
this problem and then read about another lab having the same difficulty with
Surgipath Hematoxylin starting about the same timeframe. Has Surgipath
changed something?
Charles Embrey Jr., PA(ASCP)
Histology Manager, Carle Clinic
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of DNon
Sent: Saturday, November 03, 2007 9:42 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mucin overstained with Hematoxylin in G.I. specimens
Hello Histonet,
Over the past several weeks our lab has had problems with hematoxylin
overstaining in the mucin of our gastric specimens. The degree of
hematoxylin overstaining is alarming in the mucin of the gastric specimens
but absent or negligible in other specimens. One pathologist reviewing the
slides indicated some of the overstained specimens appeared to be
inadequately fixed. We have addressed that issue which showed some
improvement, but the problem persists. We are making changes to increase
fixation time (although the specimens now appear adequately fixed yet retain
a large measure of the overstaining), but any further suggestions for
corrective action would be much appreciated.
We've been hesitant to increase acid alcohol clearing time because our
other specimens are staining well with the current protocol.
We are using the following reagents and stain times:
- Surgipath specimen containers pre-filled with 10% neutral buffered
Formalin.
- Surgipath Gills III Hematoxlyin - 6.5 minutes followed by running water
washes
- .75% HCL in 70% ETOH for 1 second (1 dip) followed by running water wash
- Surgipath Scott's Tap Water for 1 min followed by running water wash
Sakura automated stainer set to agitate ( up and down dipping of racks )
once every 2 seconds, which is the fastest speed available on it.
Dick Non
Pathology Department
Ocean County Medical Lab
Brick, New Jersey _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Mon, 5 Nov 2007 16:26:45 -0500
From: "Douglas D Deltour" <doug <@t> ppspath.com>
Subject: RE: [Histonet] Mucin overstained with Hematoxylin in G.I.
specimens
To: "'Charles.Embrey'" <Charles.Embrey <@t> carle.com>, "'DNon'"
<d.non <@t> verizon.net>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<mailman.102.1194360191.11987.histonet <@t> lists.utsouthwestern.edu>
Content-Type: text/plain; charset="US-ASCII"
Charles,
We also are having the same issue with the Surgipath Gills III. This issue
also started last month. Very interesting.
Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
Office (803)252-1913
Fax (803)254-3262
Doug <@t> ppspath.com
*****************************************************
PROFESSIONAL PATHOLOGY SERVICES, PC
NOTICE OF CONFIDENTIALITY
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Charles.Embrey
Sent: Monday, November 05, 2007 4:09 PM
To: DNon; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Mucin overstained with Hematoxylin in G.I. specimens
I find this post most interesting. After using Surgipath Gills III for
the past 7 years we started having a problem with blue mucin just last
month. I corrected the problem by adding GAA but I now have to monitor
the pH on a daily basis. It is strange that after all these years I
would suddenly have this problem and then read about another lab having
the same difficulty with Surgipath Hematoxylin starting about the same
timeframe. Has Surgipath changed something?
Charles Embrey Jr., PA(ASCP)
Histology Manager, Carle Clinic
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of DNon
Sent: Saturday, November 03, 2007 9:42 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mucin overstained with Hematoxylin in G.I. specimens
Hello Histonet,
Over the past several weeks our lab has had problems with
hematoxylin overstaining in the mucin of our gastric specimens. The
degree of hematoxylin overstaining is alarming in the mucin of the
gastric specimens but absent or negligible in other specimens. One
pathologist reviewing the slides indicated some of the overstained
specimens appeared to be inadequately fixed. We have addressed that
issue which showed some improvement, but the problem persists. We are
making changes to increase fixation time (although the specimens now
appear adequately fixed yet retain a large measure of the overstaining),
but any further suggestions for corrective action would be much
appreciated.
We've been hesitant to increase acid alcohol clearing time because
our other specimens are staining well with the current protocol.
We are using the following reagents and stain times:
- Surgipath specimen containers pre-filled with 10% neutral buffered
Formalin.
- Surgipath Gills III Hematoxlyin - 6.5 minutes followed by running
water washes
- .75% HCL in 70% ETOH for 1 second (1 dip) followed by running water
wash
- Surgipath Scott's Tap Water for 1 min followed by running water wash
Sakura automated stainer set to agitate ( up and down dipping of racks )
once every 2 seconds, which is the fastest speed available on it.
Dick Non
Pathology Department
Ocean County Medical Lab
Brick, New Jersey
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Mon, 5 Nov 2007 16:43:34 -0500
From: "Yaskovich, Ruth A (NIH/NIDCR) [E]"
<ryaskovich <@t> dir.nidcr.nih.gov>
Subject: RE: [Histonet] deplasticizing PMMA sections for staining
To: "John Baker" <bakerj <@t> umich.edu>,
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CDECB5092C07B74CBF59AD7C7F82197603260A6B <@t> NIHCESMLBX3.nih.gov>
Content-Type: text/plain; charset="us-ascii"
John,
You can soak them in 3 changes of acetone for 15 minutes each. Put them
in the first acetone right out of the oven.
Ruth Yaskovich
National Institutes of Health
National Institute of Dental and Craniofacial Research
-----Original Message-----
From: John Baker [mailto:bakerj <@t> umich.edu]
Sent: Monday, November 05, 2007 12:07 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] deplasticizing PMMA sections for staining
>
>
> Hello All, We are sectioning PMMA embedded rat femur at 5 microns on
> the Polycut. Then we are deplasticizing for H & E and Trichrome
> stains. It has been taking over 2 weeks with several changes of xylene
> to get them done and still some remnants of plastic there. I see in
> Sheehan's Practice and Theory of Histotechnology that in several
> protocols they deplasticize in xylene for 4 hours at 55 degrees C.
> The flash point of xylene is 25 degree C. Is it safe in covered
> staining dishes to actually do this? Also, we use Mayer's Hematoxylin
> and 2% Eosin to stain. What H & E protocol do you use for bone? Any
> suggestions on how to speed up the deplasticizing process welcome.
> Does anyone have Diane Sterchi's and Gayle Callis's email addresses,
> lost them?
> Thanks, John
>
>
>
>
>
John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
109 Zina Pitcher Place, 2218 BSRB
Ann Arbor, MI 48109-2200
734-936-1635
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Mon, 5 Nov 2007 15:45:56 -0600
From: "Bernice Frederick" <b-frederick <@t> northwestern.edu>
Subject: [Histonet] NSH
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001c81ff5$42a1aec0$d00f7ca5 <@t> lurie.northwestern.edu>
Content-Type: text/plain; charset="us-ascii"
This was my first meeting and whereas I enjoyed myself tremendously I only
had 2 gripes
1. There should be a block of time set aside to see the vendors. Most
of us were in workshops for a lot of the time and had to do quick run up to
the vendors.
2. I was surprised water was only provided for the speakers. It was so
dry I was going through 36-48 oz of water every day and at 2$ a pop... If
I'd known, I'd have run off to Walgreens or somewhere.
I do plan to be back and really enjoyed everybody and everything. Missed
meeting Joe though I thought we had arranged for that (tee hee).
Bernice
Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
------------------------------
Message: 16
Date: Mon, 05 Nov 2007 15:51:47 -0600
From: LuAnn Anderson <ander093 <@t> tc.umn.edu>
Subject: RE: [Histonet] Mucin overstained with Hematoxylin in G.I.
specimens
To: "Charles.Embrey" <Charles.Embrey <@t> carle.com>, "DNon"
<d.non <@t> verizon.net>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <smtpd.1c1f.472f9082.7500d.1 <@t> mta-w2.tc.umn.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed
I too have had problems recently with Surgipath hematoxylin in brain
tissue (not mucin). They replaced what I had with a new bottle, but I
am seeing the same problems. I believe the formula has been changed
to eliminate mercury--don't know if this is the basis of the
problems or not--but I believe that is when I started having
problems. They have a "new" hematoxylin (560) and eosin (515), but
our pathologists did not care for those either. I have been using
Surgipath for 14 years and just started having staining problems with it.
LuAnn Anderson
Neuropthology Lab
University of Minnesota
At 03:08 PM 11/5/2007, Charles.Embrey wrote:
>I find this post most interesting. After using Surgipath Gills III for
>the past 7 years we started having a problem with blue mucin just last
>month. I corrected the problem by adding GAA but I now have to monitor
>the pH on a daily basis. It is strange that after all these years I
>would suddenly have this problem and then read about another lab having
>the same difficulty with Surgipath Hematoxylin starting about the same
>timeframe. Has Surgipath changed something?
>
>Charles Embrey Jr., PA(ASCP)
>Histology Manager, Carle Clinic
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of DNon
>Sent: Saturday, November 03, 2007 9:42 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Mucin overstained with Hematoxylin in G.I. specimens
>
>Hello Histonet,
>
> Over the past several weeks our lab has had problems with
>hematoxylin overstaining in the mucin of our gastric specimens. The
>degree of hematoxylin overstaining is alarming in the mucin of the
>gastric specimens but absent or negligible in other specimens. One
>pathologist reviewing the slides indicated some of the overstained
>specimens appeared to be inadequately fixed. We have addressed that
>issue which showed some improvement, but the problem persists. We are
>making changes to increase fixation time (although the specimens now
>appear adequately fixed yet retain a large measure of the overstaining),
>but any further suggestions for corrective action would be much
>appreciated.
>
> We've been hesitant to increase acid alcohol clearing time because
>our other specimens are staining well with the current protocol.
>
>We are using the following reagents and stain times:
>
>- Surgipath specimen containers pre-filled with 10% neutral buffered
>Formalin.
>- Surgipath Gills III Hematoxlyin - 6.5 minutes followed by running
>water washes
>- .75% HCL in 70% ETOH for 1 second (1 dip) followed by running water
>wash
>- Surgipath Scott's Tap Water for 1 min followed by running water wash
>
>Sakura automated stainer set to agitate ( up and down dipping of racks )
>once every 2 seconds, which is the fastest speed available on it.
>
>
>Dick Non
>Pathology Department
>Ocean County Medical Lab
>Brick, New Jersey
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Mon, 5 Nov 2007 16:52:46 -0500
From: "FU,DONTAO" <fudo <@t> pathology.ufl.edu>
Subject: [Histonet] how to keep thick sections on the slides after
deparaffin
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<81FEA2B93DC53C4B8CF3667707CA592A0DCE8C <@t> path-exchange.ad.ufl.edu>
Content-Type: text/plain; charset="us-ascii"
Hi,
I met a big problem in keeping thick sections on the slides after
deparaffin recently. I cut a 70um-thick mouse retina section on
superfrost plus Gold slides, leaving it dry for 2days. Before
deparaffin, I put the slide in the 65C oven for 30 min to let it melt,
then I did the general deparaffin procedure. Unfortunately, the section
fell off from the slide. Does anyone have any experience on deparaffin
thick sections? Any suggestions?
Thank you,
Ann Dongtao Fu MD Ph.D
Lab Manager
Molecular Pathology core
Dept. of Pathology
University of Florida
Lab Phone: 352-273-7752
FAX: 352-273-7753
Rm: D11-50
------------------------------
Message: 18
Date: Mon, 05 Nov 2007 18:51:18 -0500
From: "DNon" <d.non <@t> verizon.net>
Subject: Re: [Histonet] Mucin overstained with Hematoxylin in G.I.
specimens
To: "Charles.Embrey" <Charles.Embrey <@t> carle.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <000601c82006$c3081ac0$1f0aa8c0 <@t> cyto.ocml.com>
Content-Type: text/plain; format=flowed; charset=iso-8859-1;
reply-type=original
I spoke with Surgipath's QC department today and asked if there was a change
in their Gill's III formulation. According to Mike Urban at Surgipath,
nothing has changed. Nonetheless, he is going to run additional tests on
the lot number in question and will get back to me. I find your post very
interesting indeed as our lab has used Surgipath Gill's III for the last 10
years.
They ( Surgipath) will also be sending me a gallon of Gill's III from a
different lot. If it doesn't show improvement, we'll either lower the ph
similar to what you did, or switch to Harris hematoxylin.
----- Original Message -----
From: "Charles.Embrey" <Charles.Embrey <@t> carle.com>
To: "DNon" <d.non <@t> verizon.net>; <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, November 05, 2007 4:08 PM
Subject: RE: [Histonet] Mucin overstained with Hematoxylin in G.I. specimens
I find this post most interesting. After using Surgipath Gills III for
the past 7 years we started having a problem with blue mucin just last
month. I corrected the problem by adding GAA but I now have to monitor
the pH on a daily basis. It is strange that after all these years I
would suddenly have this problem and then read about another lab having
the same difficulty with Surgipath Hematoxylin starting about the same
timeframe. Has Surgipath changed something?
Charles Embrey Jr., PA(ASCP)
Histology Manager, Carle Clinic
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of DNon
Sent: Saturday, November 03, 2007 9:42 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mucin overstained with Hematoxylin in G.I. specimens
Hello Histonet,
Over the past several weeks our lab has had problems with
hematoxylin overstaining in the mucin of our gastric specimens. The
degree of hematoxylin overstaining is alarming in the mucin of the
gastric specimens but absent or negligible in other specimens. One
pathologist reviewing the slides indicated some of the overstained
specimens appeared to be inadequately fixed. We have addressed that
issue which showed some improvement, but the problem persists. We are
making changes to increase fixation time (although the specimens now
appear adequately fixed yet retain a large measure of the overstaining),
but any further suggestions for corrective action would be much
appreciated.
We've been hesitant to increase acid alcohol clearing time because
our other specimens are staining well with the current protocol.
We are using the following reagents and stain times:
- Surgipath specimen containers pre-filled with 10% neutral buffered
Formalin.
- Surgipath Gills III Hematoxlyin - 6.5 minutes followed by running
water washes
- .75% HCL in 70% ETOH for 1 second (1 dip) followed by running water
wash
- Surgipath Scott's Tap Water for 1 min followed by running water wash
Sakura automated stainer set to agitate ( up and down dipping of racks )
once every 2 seconds, which is the fastest speed available on it.
Dick Non
Pathology Department
Ocean County Medical Lab
Brick, New Jersey
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 19
Date: Tue, 6 Nov 2007 08:24:24 -0000
From: "Kemlo Rogerson" <Kemlo.Rogerson <@t> waht.swest.nhs.uk>
Subject: RE: [Histonet] how to keep thick sections on the slides after
deparaffin
To: "FU,DONTAO" <fudo <@t> pathology.ufl.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<86ADE4EB583CE64799A9924684A0FBBF0222F0E6 <@t> wahtntex2.waht.swest.nhs.uk>
Content-Type: text/plain; charset="us-ascii"
Hi,
I met a big problem in keeping thick sections on the slides after
deparaffin recently. I cut a 70um-thick mouse retina section on
superfrost plus Gold slides, leaving it dry for 2days. Before
deparaffin, I put the slide in the 65C oven for 30 min to let it melt,
then I did the general deparaffin procedure. Unfortunately, the section
fell off from the slide. Does anyone have any experience on deparaffin
thick sections? Any suggestions?
Thank you,
You could use bovine albumin to coat the slides (I think 1% but I'm not
sure) then dry at 37 degrees for those two days then into the oven at 60
degrees (ish). Think that sort of sets the section onto the slide but
you need to get the concentration correct as the protein could stain
with some techniques if its too concentrated.
Kemlo Rogerson
Pathology Manager
DD 01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;
No snowflake in an avalanche ever feels responsible. --George Burns
This e-mail is confidential and privileged. If you are not the intended
recipient please accept my apologies; please do not disclose, copy or
distribute information in this e-mail or take any action in reliance on
its contents: to do so is strictly prohibited and may be unlawful.
Please inform me that this message has gone astray before deleting it.
Thank you for your co-operation
------------------------------
Message: 20
Date: Tue, 06 Nov 2007 10:07:04 GMT
From: "Barbara Webb" <barbwebb <@t> webtv.net>
Subject: [Histonet] FYI - Brainbow
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <BAY137-DAV1112917A32FD0C5CC5AADBD8890 <@t> phx.gbl>
Content-Type: text/plain; charset="utf-8"
Looking at the fuure?
http://www.nytimes.com/2007/11/06/health/research/06brai.html?_r=1&ref=scien
ce&oref=slogin
------------------------------
Message: 21
Date: Tue, 6 Nov 2007 05:20:07 -0500
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: RE: [Histonet] Histo, cyto job in NY
To: <Xorren <@t> aol.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000801c8205e$9b559130$0202a8c0 <@t> HPPav2>
Content-Type: text/plain; charset="us-ascii"
If you are looking for a job as a histotech in New York, it's not going to
happen. The NY legislators have made a licensure law that only certified med
techs can be histotechs. Those already working as histotechs in NY can
continue to work in NY. However, any certified histotech moving into NY
cannot work as a histotech until they take all the med tech courses and pass
the med tech certification exam. NY will not recognize the NAACLS histotech
program graduates from SUNY, the only accredited HT program in NY. (SUNY
will be closing, as it's impossible to offer all med tech courses and all
histotech courses, all in a 2 year program.) NY will not recognize those
earning their ASCP HT or HTL certification through the associate degree
on-the-job training route.
ASCP, NY histotech society, and the NY pathologist groups are lobbying for
changes, but the legislators are not listening. NSH has weighed in with
ASCP, and the NY pathologists are coming to NSH to see what NSH can do, too.
(That's where Vinnie DelaSperanza's (NSH presidents) comments on needing
more members come into play. If ASCP and pathologist groups aren't swaying
the legislators, then NSH with our fewer numbers are not going to help.
Histotechs need to join NSH and ASCP, to histotechs have more numbers and
will be listened to better.)
The original draft of the bill, according to Vinnie, included histotechs
with the appropriate information on training and certification and job
responsibilities. The bill got changed over the years, and went unnoticed by
NY histotechs. The bill took years (decade+) to pass, so the legislators
don't want to change anything on the law at this stage. It may take years to
correct.
At this time, the med tech programs do not have classes in the theory or
practical aspects of histotechnology. I don't know if they are planning on
adding them to the med tech curriculum.
In the mean time, NY histotechs will be retiring and leaving the field.
Workloads will increase as the patient populations ages and technology
requires more tissue tests. And there will be fewer and fewer NY histotechs
to do these jobs.
It's a histology crisis in the making.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Xorren <@t> aol.com
Sent: Monday, November 05, 2007 1:44 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Histo, cyto job in NY
Hello all !
Does anyone know of any histology or cytology prep job in the NY area.
I have have 15 years in the field and hope someone has some info.
you can respond @ xorren <@t> aol.com or call (516) 671-0907 have a great day
!!!!!
**************************************
See what's new
at http://www.aol.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 22
Date: Tue, 6 Nov 2007 08:21:20 -0500
From: "Pam Barker" <relia1 <@t> earthlink.net>
Subject: [Histonet] RELIA's Histology Opportunities Update 11-06-07
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <E1IpOMp-0005gL-Lr <@t> elasmtp-junco.atl.sa.earthlink.net>
Content-Type: text/plain; charset="iso-8859-1"
Hi Histonetters!
I have been reading some of the posts on the NSH in Denver. What about
everybody else, did you make it to the NSH in Denver? If so how was it?
What was your favorite thing about it? I havent been since the
convention in 2005 in Ft. Lauderdale. Did you have a get together for
the histonet? We did at the Ft. Lauderdale convention it was a small
turnout but we had fun. I really enjoyed meeting people, learning new
things and the party at the Hard Rock Casino. I am planning on
attending the 2008 meeting in Pittsburgh. Will I see you there?
Here is a list of my current openings all of the positions are full time
permanent positions. They are M-F dayshift opportunities and my clients
offer excellent compensation, benefits and relocation assistance. And
they are ready to interview and hire right away.
****RELIA Spotlight Opportunities****
I am working with a client in the Philadelphia, PA area that has 2
unique and exciting opportunities. These are nonclinical positions.
BioSciences Product Manager
Histologist Technical and Field Support
*********************************
Histology Managers/Supervisors needed in:
Dallas, TX
Austin, TX
Philadelphia, PA
Valencia, CA
Cape Cod, MA
Histotechnicians/Histotechnologists needed in:
Phoenix, AZ
Atlanta, GA
San Francisco, CA
Los Angeles, CA
Valencia, CA
Orlando, FL
St. Petersburg, FL
Fredericksburg, VA
Dallas, TX
Waco, TX
Austin, TX
Philadelphia, PA
Pittsburgh, PA
If you know of anyone else who might be interested in any of these
positions or might like to subscribe to RELIAs Histology Careers
Bulletin please feel free to pass this e-mail along to them.
Remember it never hurts to look!!!
Thanks, Pam 877-60 RELIA (877-607-3542)
Thank You!
Pam Barker
President
RELIA
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: relia1 <@t> earthlink.net
<http://home.earthlink.net/~relia1>
www.myspace.com/pamatrelia
------------------------------
Message: 23
Date: Tue, 6 Nov 2007 06:41:31 -0800
From: "James Watson" <jwatson <@t> gnf.org>
Subject: RE: [Histonet] Histo, cyto job in NY
To: <lpwenk <@t> sbcglobal.net>, <Xorren <@t> aol.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F5A26DAD36F60843830631774C95CAE202F78E57 <@t> EXCH2.rec.gnf.org>
Content-Type: text/plain; charset="iso-8859-1"
At NSH we were told by someone that was in contact with the legistators that
wrote the bill that this did not pass and that they were rewriting the bill.
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Lee & Peggy
Wenk
Sent: Tue 11/6/2007 2:20 AM
To: Xorren <@t> aol.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Histo, cyto job in NY
If you are looking for a job as a histotech in New York, it's not going to
happen. The NY legislators have made a licensure law that only certified med
techs can be histotechs. Those already working as histotechs in NY can
continue to work in NY. However, any certified histotech moving into NY
cannot work as a histotech until they take all the med tech courses and pass
the med tech certification exam. NY will not recognize the NAACLS histotech
program graduates from SUNY, the only accredited HT program in NY. (SUNY
will be closing, as it's impossible to offer all med tech courses and all
histotech courses, all in a 2 year program.) NY will not recognize those
earning their ASCP HT or HTL certification through the associate degree
on-the-job training route.
ASCP, NY histotech society, and the NY pathologist groups are lobbying for
changes, but the legislators are not listening. NSH has weighed in with
ASCP, and the NY pathologists are coming to NSH to see what NSH can do, too.
(That's where Vinnie DelaSperanza's (NSH presidents) comments on needing
more members come into play. If ASCP and pathologist groups aren't swaying
the legislators, then NSH with our fewer numbers are not going to help.
Histotechs need to join NSH and ASCP, to histotechs have more numbers and
will be listened to better.)
The original draft of the bill, according to Vinnie, included histotechs
with the appropriate information on training and certification and job
responsibilities. The bill got changed over the years, and went unnoticed by
NY histotechs. The bill took years (decade+) to pass, so the legislators
don't want to change anything on the law at this stage. It may take years to
correct.
At this time, the med tech programs do not have classes in the theory or
practical aspects of histotechnology. I don't know if they are planning on
adding them to the med tech curriculum.
In the mean time, NY histotechs will be retiring and leaving the field.
Workloads will increase as the patient populations ages and technology
requires more tissue tests. And there will be fewer and fewer NY histotechs
to do these jobs.
It's a histology crisis in the making.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Xorren <@t> aol.com
Sent: Monday, November 05, 2007 1:44 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Histo, cyto job in NY
Hello all !
Does anyone know of any histology or cytology prep job in the NY area.
I have have 15 years in the field and hope someone has some info.
you can respond @ xorren <@t> aol.com or call (516) 671-0907 have a great day
!!!!!
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End of Histonet Digest, Vol 48, Issue 6
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