[Histonet] deplasticizing PMMA sections for staining
PMonfils <@t> Lifespan.org
Mon Nov 5 13:22:08 CST 2007
In my experience, xylene is a rather poor solvent for PMMA. It works much faster at 60 degrees, but don't try heating flammable solvents in your oven unless you are certain it is certified explosion-proof (most paraffin ovens are not). Even then, unless you have the oven in or at least next to a fume hood, you'll get a lot of xylene vapors in the air as a result of heating it. I deplasticise PMMA with 2-methoxyethyl acetate, which only takes a couple of hours at room temperature. This has a rather strong though not unpleasant odor, and should be used in the hood.
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