[Histonet] deplasticizing PMMA sections for staining
bakerj <@t> umich.edu
Mon Nov 5 11:07:07 CST 2007
> Hello All, We are sectioning PMMA embedded rat femur at 5 microns on
> the Polycut. Then we are deplasticizing for H & E and Trichrome
> stains. It has been taking over 2 weeks with several changes of xylene
> to get them done and still some remnants of plastic there. I see in
> Sheehan's Practice and Theory of Histotechnology that in several
> protocols they deplasticize in xylene for 4 hours at 55 degrees C.
> The flash point of xylene is 25 degree C. Is it safe in covered
> staining dishes to actually do this? Also, we use Mayer's Hematoxylin
> and 2% Eosin to stain. What H & E protocol do you use for bone? Any
> suggestions on how to speed up the deplasticizing process welcome.
> Does anyone have Diane Sterchi's and Gayle Callis's email addresses,
> lost them?
> Thanks, John
John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
109 Zina Pitcher Place, 2218 BSRB
Ann Arbor, MI 48109-2200
More information about the Histonet