[Histonet] rat brain parafin embedding

[dimas]

Hello All.

  I am a student and I have problems whith rat brain 
paraffin embedding. To do good slices was very difficult.
I tried to find protocols, but anywhere is  wrote that 
brains were dehydrated throught a graded series of 
ethanol, clear in xylene and embedded according standard 
protocol.
If anyone else has experience with rat brain paraffin 
embedding,could you send
protocol.
If it is not so difficult could you send fotos or just 
whrite how to make slices 1-2mm thick in order to 
orientation in atlas, and how brains embedded.

Dmitriy Lanshakov
Novosibirsk State University
student
flip-flop <@t> ngs.ru
ssdd <@t> gorodok.net