[Histonet] Veronal-acetate buffer

[Jacqui Detmar]

Hi all.  I would like to do some acid phosphatase staining, but the
protocol I have includes a buffer that calls for sodium barbitone.  I
have searched a number of different sites for a possible substitute
(including Histonet archives) and I can't seem to find one.  Does anyone
know if I can substitute another buffer for the veronal-acetate buffer?


 

Thanks,

 

Jacqui Detmar, Ph.D. candidate

Samuel Lunenfeld Research Insitute, room 876

Mount Sinai Hospital

600 University Avenue

Toronto, ON, Canada

M5G 1X5