[Histonet] vacuoles in H&E stain

[Hugh Luk]

This was my first impression also.  If your temperature is around 100 C, you 
are splitting the nuclear contents.

Jackie O'Conner and I have seen it once before, from a reference lab we were 
using.

The solution is simple: you just need to bring your dryer temperature down 
(lessening the temperature should increase your drying time).

The only other thing that comes to mind is too much water in your fixative 
(hypotonic), but your experiment with the lab across town, discounted this.

Good luck,
Hugh Luk, HTL (ASCP)
hluk <@t> crch.hawaii.edu
Pathology Shared Resources Lab
Cancer Research Center of Hawaii

>From: Rene J Buesa <rjbuesa <@t> yahoo.com>
>To: "Elgert, Phil" <elgerp <@t> kadlecmed.org>, 
>histonet <@t> lists.utsouthwestern.edu
>Subject: Re: [Histonet] vacuoles in H&E stain
>Date: Fri, 25 May 2007 12:25:05 -0700 (PDT)
>
>The dryer is too hot!
>   René J.
>
>"Elgert, Phil" <elgerp <@t> kadlecmed.org> wrote:
>   Hi All,
>
>
>
>Here's a stumper. We deparaffinize for 10 minutes. We use Richard Allen
>hematolylin and eosin. We have 5 alcohols before going into our last
>Xylenes for clearing. So no water at the end. We still have a lot of
>nuclear vacuoles on the finished slides. I suspected processing but when
>I sent blocks to a lab across town they turned out fine. Has anybody
>seen this before?
>
>
>
>p.s. Have a great memorial day weekend.
>
>
>
>Phil Elgert
>
>Histology Supervisor
>
>Kadlec Medical Center
>
>888 Swift Blvd.
>
>Richland, Wa 99352
>
>
>
>
>
>
>
>
>
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