[Histonet] vacuoles in H&E stain

[Rene J Buesa]

The dryer is too hot!
  René J.

"Elgert, Phil" <elgerp <@t> kadlecmed.org> wrote:
  Hi All,



Here's a stumper. We deparaffinize for 10 minutes. We use Richard Allen
hematolylin and eosin. We have 5 alcohols before going into our last
Xylenes for clearing. So no water at the end. We still have a lot of
nuclear vacuoles on the finished slides. I suspected processing but when
I sent blocks to a lab across town they turned out fine. Has anybody
seen this before? 



p.s. Have a great memorial day weekend.



Phil Elgert

Histology Supervisor

Kadlec Medical Center

888 Swift Blvd.

Richland, Wa 99352









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