[Histonet] Re: Ethanol ready-to-use for LCM

[Laura Harris]

Hi Luminita

Ambion might sell this, if they don't then it is probably not available 
as a specific product. RNases do not generally survive in 100% ethanol 
so the ethanol itself does not need to be special. We use the PALM 
system and have had good results making up our own ethanols with 
RNase-free water.

Cheers, Laura

Institute of Biotechnology, University of Cambridge, UK.

Date: Wed, 23 May 2007 10:28:19 -0400
 > From: "Castillos, Luminita" <lcastillos <@t> cogenics.com>
 > Subject: [Histonet] Ethanol ready-to-use for LCM
 > To: <histonet <@t> lists.utsouthwestern.edu>
 > Message-ID:
 > 	<DE517009A0E1B345BAE029F86CADECCB059934 <@t> ncmsx2.gpi.genaissance.com>
 > Content-Type: text/plain;	charset="US-ASCII"
 >
 >
 > Hi,
 >
 > Does any know from where to buy ready-to-use ethanol (100%, 95%, 70%)
 > molecular biology grade and RNase-free, compatible for Laser Capture
 > microdissection staining protocol?. Thank you very much.
 >
 > L,