[Histonet] Re: Histonet Digest, Vol 42, Issue 26

Sun May 20 07:46:56 CDT 2007

Hi,  I do special satins on frozen muscle sections sections. I air dry them for about one hour and never fix them. try it and tell me how it works out.

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Today's Topics:

1. Can you top this? (Margaret Horne)
2. Please remove (mjlco <@t> aol.com)
3. frozen special stains (Ingles Claire)
4. Help with Tunel (Michael J. Lyon, Ph.D.)
5. Flex Alcohols (Marsh, Nannette)
6. Re: Processing (Joe Nocito)
7. RE: Friday Fun Fume (Edwards, R.E.)
8. Re: RE: [Histonet] Friday Fun Fume (histology <@t> gradymem.org)
9. Re: RE: [Histonet] Friday Fun Fume (Lynette Pavelich)
10. RE: RE: [Histonet] Friday Fun Fume (Shirley Powell)
11. RE: [SPAM] Re: RE: [Histonet] Friday Fun Fume (Mickie Johnson)
12. Can You Beat This? (Breeden, Sara)
13. CSI Las Vegas (Jackie M O'Connor)
14. RE: CSI Las Vegas (Douglas D Deltour)
15. RE: Can You Beat This? (Weems, Joyce)
16. Re: CSI Las Vegas (Akemi Allison-Tacha)
17. RE: CSI Las Vegas (Shirley Powell)
18. Re: CSI Las Vegas (Thomas Pier)

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Message: 1
Date: Thu, 17 May 2007 14:45:23 -0400
From: "Margaret Horne" 
Subject: [Histonet] Can you top this?
To: 
Message-ID: <464C6A83.23366.88628D <@t> localhost>
Content-Type: text/plain; charset=US-ASCII

I keep my diluted antibodies in shell vials but needed a way to 
keep them organised, so :

I got a Ferrraro Roche container from a friend ( clear plastic , 
rectangular) and the cardboard divider from a - 80 freezer box. Cut 
the divider to fit and organised the vials in their serial dilutions. Fits 
beautifully in the door of the fridge. A second similar container 
holds my small bottles of streptavidin and my Biotinylated 
secondary, etc. My friend thinks I should get another box of those 
chocolates and charge it to my boss. I haven't had the nerve.

My incubation chambers for IHC are rubbermaid containers.

For TEM IHC I got two ceramic escargo dishes, each has 6 wells, 
for $2 in a grocery store sale bin. I was tickled as the ones from an 
EM catalogue are about $15 each.

But my favourite is the wooden stir stick with a eyelash attached to 
the end by a dab of nail polish that I use with my $3000 diamond 
knife to do TEM work. The incongruity always makes me chuckle.

I like this thread. It's more than a good chuckle. It's an 
exchange of neat ideas.
thanks , margaret
Margaret Horne ,
Histology Teaching Assistant,
Dept. of B.SC.,
Atlantic Veterinary College, U.P.E.I.,
550 University Ave., Charlottetown,
P.E.I., C1A 4P3 
Canada

------------------------------

Message: 2
Date: Thu, 17 May 2007 14:49:51 -0400
From: mjlco <@t> aol.com
Subject: [Histonet] Please remove
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8C966CB491EAE11-16D8-1665 <@t> webmail-db17.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

I am off on holiday and dont want to fill up my box.
Thanks,
matt
Longmont united hospital
________________________________________________________________________
AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com.

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Message: 3
Date: Thu, 17 May 2007 14:42:28 -0500
From: "Ingles Claire" 
Subject: [Histonet] frozen special stains
To: 
Message-ID:
<08A0A863637F1349BBFD83A96B27A50A120042 <@t> uwhis-xchng3.uwhis.hosp.wisc.edu>

Content-Type: text/plain; charset="iso-8859-1"

Hello out there!
I have a question for the collective histo-brain out there. (resistance is futile). Does anyone do special stains i.e. trichrome and/or elastic stains on frozen tissue (skin)? I have been trying these out using the regular protocols you would use for FFPE tissues. The (FFPE) controls I stain with the frozens come out fine, but the staining of the frozen sections is so intense that it is difficult to interpret. On the trichrome, the epidermis is so dark it looks black. I've tried cutting the incubation times back by as much as half. It helps a bit but is still pretty intense. Are there any special procedures out there to make them turn out more normal looking? For procedure, I cut sections at 5 microns, dry in a 60 oven for 10 min, and fix in 10% formalin for 30 min. Then rinse in running tap for about 5 minutes and perform stain as normal. Help?!?

Claire Ingles
UW Hospital
Madison WI

------------------------------

Message: 4
Date: Thu, 17 May 2007 15:37:09 -0400
From: "Michael J. Lyon, Ph.D." 
Subject: [Histonet] Help with Tunel 
To: 
Message-ID: <004a01c798ba$c2a571f0$31ae7f8b <@t> lyonoffice>
Content-Type: text/plain; charset="us-ascii"

I am looking for some help using the Chemicon ApopTag kit. We have used
this successfully in the past on formalin fixed human muscle, frozen
sections. We are looking at a different human muscle, processed similarly.
However our results are incredibly inconsistent even on the positive control
DNAse treated sections of the same muscle. Some of the sections have
nothing at all. While other sections have areas of very bright nuclei and
other areas are nearly negative. 

Any help appreciated.

Thanks

Michael J. Lyon, Ph.D.

Otolaryngology Research Lab

SUNY Upstate Medical University

750 East Adams Street

Syracuse, NY 13210

Voice 315-464-7253

Fax 315-464-5572

------------------------------

Message: 5
Date: Thu, 17 May 2007 15:30:37 -0500
From: "Marsh, Nannette" 
Subject: [Histonet] Flex Alcohols
To: 
Message-ID:

Content-Type: text/plain; charset="us-ascii"

Hello. We are considering switching to the Flex alcohols produced by
Richard Allen. Any comments, especially from research personnel are
greatly appreciated. Thanks, Nanne

Nanne Marsh 
Histology Specialist I
Stowers Institute for Medical Research

------------------------------

Message: 6
Date: Tue, 22 May 2007 18:09:49 -0500
From: "Joe Nocito" 
Subject: Re: [Histonet] Processing
To: "Douglas D Deltour" , "'Jackie M O'Connor'"
, "'Coco'" 
Cc: Histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID: <015a01c79cc6$4ef49d20$d49eae18 <@t> yourxhtr8hvc4p>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original

Heather,
have you tried talking to Civilian Personnel? I had a GS7 who was a 
force-hire because another base closed due to BRAC. You talk about a 
nightmare! Freddy Kruger had nothing on this woman. Civilian Personnel was a 
saving grace.

JTT
----- Original Message ----- 
From: "Douglas D Deltour" 
To: "'Jackie M O'Connor'" ; "'Coco'" 

Cc: ; 

Sent: Wednesday, May 16, 2007 1:54 PM
Subject: RE: [Histonet] Processing

>I agree with Jackie on this Heather. Having worked in an exact environment
> that you work in (A Navy Hospital) I can tell you that the military and 
> the
> GS have a totally different mindset than the "real world". I did it for 14
> years (I won't tell you which side). Just face up to it because you will
> always have some sort of a military vs. GS conflict. You can try to rattle
> the military paths cage but to him you will be "the civilian" and if you
> make too many waves then you will be a "trouble making civilian".
> It sounds like you do not have a very supportive GS chain at your 
> hospital.
> Most likely your GS boss is on the clinical side and considers the AP side
> the "stepchild" of the lab. Maybe he/she lacks the backbone to support 
> you.
> It is all about getting through another day without any bother for most.
>
> As for the GS PD... I bet you and your worker have a little sentence at 
> the
> end of it that reads "All other duties assigned", or something like it.
>
> The GS pay system is below standard and the benefits are not what they 
> used
> to be. Is it really worth the headache?
>
> No offense to the GS workers out there. I appreciate your tolerance.
>
>
> Douglas D. Deltour HT(ASCP)
> Histology Manager
> Professional Pathology Services, PC
> One Science Court
> Suite 200
> Columbia, SC 29203
> (803)252-1913
> Fax (803)254-3262
>
> *****************************************************
> PROFESSIONAL PATHOLOGY SERVICES, PC
> NOTICE OF CONFIDENTIALITY
> This message is intended only for the use of the individual or entity to
> which it is addressed and may contain information that is privileged,
> confidential and exempt from disclosure under applicable law. If the 
> reader
> of this message is not the intended recipient, you are hereby notified 
> that
> any dissemination, distribution, or copying of this communication is
> strictly prohibited by law. If you have received this communication in
> error, please notify me immediately.
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jackie M
> O'Connor
> Sent: Wednesday, May 16, 2007 7:44 AM
> To: Coco
> Cc: Histonet <@t> lists.utsouthwestern.edu;
> histonet-bounces <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Processing
>
> Heather -
> If you're not active duty, why don't you get out of that job? Seems like
> you've been struggling a long time there - -maybe that job just isn't the
> right fit.
> Best wishes,
> Jackie
>
>
>
> "Coco" 
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 05/15/2007 06:13 PM
>
> To
> 
> cc
>
> Subject
> [Histonet] Processing
>
>
>
>
>
>
> Well anybody who is a GS, if you know a friend of a friend, they will
> get
> you a job. I agree military believe that civilians are enlisted people,
> and
> think that you can be cross trained in every department, like a corpsman.
> Unfortunately, that is why we have work pd's. Civilian vs. military is not
> a
> great interface. A lot of problems are not dealt with until they directly
> affect that person. Anyways, to the person who said, "report it to CAP".
> What will they do? At this point, I wish it was easy to say that this
> person
> could label slides. The accessioning process after 60 days is a nightmare.
> I've repeated, visually shown, written things down and this lady is still
> struggling. But for those who are not GS. A GS 7 can not train a GS 7, nor
> the secretary at GS 5, can not train a GS 7. The work PD does not state
> OJT.
> So right now there is a major violation going on. This lady just happened
> to
> want a GS job so badly, she might have sold her soul to the devil to get
> it
> and now she is paying dearly. So she is on a year probation, and I'm
> between
> a rock and a hard place because she is not capable of performing the job.
> I
> have already rattled the pathologists cage, and I am getting ignored. I'm
> just as guilty of standing by and doing nothing when patient care is being
> compromised and when there is a problem, military come after the civilian
> and pin the blame on you. That is why the BS is unreal and very very
> political. I am debating whether to tell the CO. What should I do? That is
> a
> trickle down effect and there will be retaliation. Anybody have any ideas
> on
> what I should do? Let it go, or go to the top and than commanders will
> take
> some serious heat. Regardless oh how this situation is dealt with the
> nending is not going to be nice. I don't want this woman to lose her job
> but
> than again she is incompetent. All opinions appreciated.
>
>
>
> Heather
>
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>
>
>
>
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------------------------------

Message: 7
Date: Fri, 18 May 2007 10:10:07 +0100
From: "Edwards, R.E." 
Subject: RE: [Histonet] Friday Fun Fume
To: "Poteete, Jacquie A." , "Breeden,
Sara" , 
Message-ID:

Content-Type: text/plain; charset="US-ASCII"

When I was a lad we used an electric iron as a hot plate
and a toaster to dry the slides in, and warm up our meals, and
dry our wet clothes on top of the autoclave never did me any
harm! 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Poteete,
Jacquie A.
Sent: 17 May 2007 15:44
To: Breeden, Sara; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Friday Fun Fume

We're close to that. Our main IHC work area is an old shelf placed
across the top of two open drawers.

Jacquie Poteete MT(ASCP)QIHC
Tulsa, OK

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Thursday, May 17, 2007 8:48 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Friday Fun Fume

I'm back from hibernation and have an idea for a new end-of-week grin
(hopefully). It's called "Can You Beat This?". Here's mine: I do
special stains in an 18x18" "wet bar" sink using an under-counter
refrigerator rack as my slide support. Can you beat that? Knock
yourself out...

Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 700

Albuquerque, NM 87106

505-841-2576

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------------------------------

Message: 8
Date: Fri, 18 May 2007 07:20:01 -0500
From: histology <@t> gradymem.org
Subject: Re: RE: [Histonet] Friday Fun Fume
To: "Edwards, R.E." 
Cc: histonet <@t> lists.utsouthwestern.edu, "Breeden, Sara"

Message-ID: 
Content-Type: text/plain; charset=us-ascii

When they first opened a pathology lab here (30+ yrs ago) the 
histology lab was in a former bathroom. You could do gross, put it in 
the processor, embed, cut, and stain just by swiveling the chair 
around. And you had to go through the pathologist's office to enter 
and leave the "lab"...and he was quite a bear in those days!!

Angie Barnett, HTL(ASCP)
Grady Memorial Hospital
Pathology Department
405/224-2258
histology <@t> gradymem.org

----- Original Message -----
From: "Edwards, R.E." 
Date: Friday, May 18, 2007 4:17 am
Subject: RE: [Histonet] Friday Fun Fume
To: "Poteete, Jacquie A." , "Breeden, 
Sara" , histonet <@t> lists.utsouthwestern.edu

> When I was a lad we used an electric iron as a hot plate
> and a toaster to dry the slides in, and warm up our 
> meals, and
> dry our wet clothes on top of the autoclave never did me 
any
> harm! 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
> Poteete,Jacquie A.
> Sent: 17 May 2007 15:44
> To: Breeden, Sara; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Friday Fun Fume
> 
> We're close to that. Our main IHC work area is an old shelf placed
> across the top of two open drawers.
> 
> Jacquie Poteete MT(ASCP)QIHC
> Tulsa, OK
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
> Breeden,Sara
> Sent: Thursday, May 17, 2007 8:48 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Friday Fun Fume
> 
> 
> I'm back from hibernation and have an idea for a new end-of-week grin
> (hopefully). It's called "Can You Beat This?". Here's mine: I do
> special stains in an 18x18" "wet bar" sink using an under-counter
> refrigerator rack as my slide support. Can you beat that? Knock
> yourself out...
> 
> 
> 
> Sally Breeden, HT(ASCP)
> 
> NM Dept. of Agriculture
> 
> Veterinary Diagnostic Services
> 
> PO Box 700
> 
> Albuquerque, NM 87106
> 
> 505-841-2576
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 

------------------------------

Message: 9
Date: Fri, 18 May 2007 09:25:24 -0400
From: "Lynette Pavelich" 
Subject: Re: RE: [Histonet] Friday Fun Fume
To: ,
Cc: histonet <@t> lists.utsouthwestern.edu, sbreeden <@t> nmda.nmsu.edu
Message-ID: <464D7105020000EE00014376 <@t> smtp-gw.hurleymc.com>
Content-Type: text/plain; charset=US-ASCII

Back in '71, when I was a student, we were in 1 room with 2 windows on
the same side of the building so there was no cross venilation. The box
fan was propped up in the window in front of the docs when they grossed
blowing out, winter or summer. I still remember going home so "high"
from the xylene fumes that it took about 30 minutes to come around. 
Never remembered driving home and if I ever went through a red light or
not!!! Some guardian angel, huh!!

Lynette Pavelich, HT(ASCP)
Histology Supervisor
Hurley Medical Center
One Hurley Plaza
Flint, MI 48503

ph: 810-257-9948
fax: 810-762-7082

------------------------------

Message: 10
Date: Fri, 18 May 2007 10:29:22 -0400
From: Shirley Powell 

Subject: RE: RE: [Histonet] Friday Fun Fume
To: 'Lynette Pavelich' ,
histology <@t> gradymem.org, ree3 <@t> leicester.ac.uk
Cc: histonet <@t> lists.utsouthwestern.edu, sbreeden <@t> nmda.nmsu.edu
Message-ID: <01MGQ7YQSQBK8WWLGQ <@t> Macon2.Mercer.edu>
Content-Type: text/plain; charset=us-ascii

45 years ago (coming this July 18th) when I began training (I was 2 at the
time :) ), it was in the basement of the hospital in the animal room where
they used to keep the rabbits for the pregnancy tests, no windows, not much
ventilation if any, and we dried our slides with a hair dryer (yes they had
those back then) and we stored all the specimens in jars of formalin in the
closet next to that room, so now you know why I am defective, or is it just
fixed. Xylene fumes, I can't smell anymore and my liver enzymes run high,
not me. Oh well that could be from the wine consumption. My salary at that
time would not buy groceries today for a week and gas is out of the
question. Call me an antique, not old.

Shirley Powell 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lynette
Pavelich
Sent: Friday, May 18, 2007 9:25 AM
To: histology <@t> gradymem.org; ree3 <@t> leicester.ac.uk
Cc: histonet <@t> lists.utsouthwestern.edu; sbreeden <@t> nmda.nmsu.edu
Subject: Re: RE: [Histonet] Friday Fun Fume

Back in '71, when I was a student, we were in 1 room with 2 windows on the
same side of the building so there was no cross venilation. The box fan was

=== message truncated ===

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