[Histonet] FOX P3 antibody

Gayle Callis gcallis <@t> montana.edu
Mon May 7 14:23:08 CDT 2007


The following was copied from eBioscience website.  The photographs they 
showed were murine spleen/frozen section and also human tonsil.

The Foxp3 antibodies have been validated for intracellular staining with 
flow cytometric analysis. The protocols and staining buffers have been 
developed for optimal and consistent staining Foxp3 staining while 
preserving surface antigen staining. Furthermore, the protocol was designed 
for robustness, reproducibility, ease-of-use, as well as flexibility, to 
allow researchers to stain at their convenience. In addition, the 
antibodies have been reported useful for immunohistological staining (both 
frozen and paraffin sections) and western blotting of endogenous protein 
from total human PBMCs or mouse splenocytes, without the need for 
enrichment of Tregs.
Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610






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