[Histonet] DAB & Masson's compatability

[Geoff McAuliffe]

I would think it would be fine as long as you avoid strong acids. Try it 
on a spare slide before doing the important stuff. Also, intensifying 
the DAB with nickel, nickel+cobalt, osmium vapors, silver or a 
commercial product like Vector's Intense would be worth a try.

Geoff


Walters, Katherine S wrote:

>Question-Will a slide stained with DAB hold up to a Masson's trichrome
>stain?  I am worried about losing the DAB precipitate.  Specifically, I
>want a stain that will demonstrate fibrosis and a cytokine antibody on
>the same slide.  Does anyone have any ideas about how to do this?
>TIA,
>Kathy
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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