[Histonet] DAB & Masson's compatability

Liz Chlipala liz <@t> premierlab.com
Fri Mar 30 15:32:49 CDT 2007


I have not personally tried a trichrome, but I have done Acid Fast, Cresyl
echt violet, iron, etc on DAB stained slides.  We have ran both the IHC
first and on other instances we have ran the special first (iron with CD68
IHC with Fast Red Chromagen).  Just run your immuno as usual and then rinse
in distilled water and follow with the trichrome, the only thing that I'm
not sure of is if the DAB will survive the bouins, but I think it might,
since I can't think of anything that will remove DAB from a slide.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz <@t> premierlab.com
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Walters,
Katherine S
Sent: Friday, March 30, 2007 2:02 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] DAB & Masson's compatability

Question-Will a slide stained with DAB hold up to a Masson's trichrome
stain?  I am worried about losing the DAB precipitate.  Specifically, I
want a stain that will demonstrate fibrosis and a cytokine antibody on
the same slide.  Does anyone have any ideas about how to do this?

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