[Histonet] A hepatoma cell line grow in mouse liver, How can I distinguish these two cells?

[koellingr <@t> comcast.net]

Yan Gao,

This is a great way to do it as has been described by Jackie with the one caveat that even if you are doing this with SCID mice, remember that you can have some background problems depending on the genetic background of the SCID.  B & T cell "leakage" in SCID's is a well known fact due to some small amount of residual immunocompentant activity.  Indeed, plasma Ig can be found in SCIDS, so B cells can certainly be there.  Depends on the genetic background (CB.17, C3H, ICR, etc).  Some are very clean (no leakage) so you would not have to worry about putting a mouse monoclonal on a SCID when using xenografts.  But some are very "leaky" and so mouse Ig antibodies will give you a bit of a problem.  Depends on your mouse model/background strain.

Ray Koelling
Phenopath Laboratories
Seattle, WA

-------------- Original message -------------- 
From: "Jackie M O'Connor" <Jackie.O'Connor <@t> abbott.com> 

> Human mitochondria marker will stain only human cells. 
> Chemicon - catalog number MAB1273. Your mouse is immunocompromised, i.e., 
> SCID? No need to block mouse Ig to use this mAB. 
> I use this constantly to distinguish human tumor xenografts in mice. 
> 
> 
> 
> "yan gao" 
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu 
> 03/19/2007 08:49 AM 
> 
> To 
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> Subject 
> [Histonet] A hepatoma cell line grow in mouse liver, How can I 
> distinguish these two cells? 
> 
> 
> 
> 
> 
> 
> 
> Hi, Histonet. 
> 
> We have a human hepatoma cell line injected into mouse liver. The 
> cell line grown in mouse liver for three weeks. The human hepatoma 
> cells mixed into mouse liver. Does anyone have any idea how to 
> distinguish the human hepatoma cell line in the mouse liver? What 
> marker I should use to distinguish two kinds of cells? Thanks. 
> 
> 
> Yan Gao 
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