[Histonet] tape transfer paraffin question
Thom Jensen
tissuearray <@t> hotmail.com
Tue Mar 27 07:24:10 CDT 2007
The tape transfer system often leaves a resin on the slides. If folks
don't soak the tape in the solution that removes the tape then you
will get left over glue and possibly tape left on the slide. I don't
recommend the tape method for cutting array blocks. It is mostly used
by techs who are either inexperienced or have a hard time cutting
array blocks. The samples are often uninterpretable because of the
Tape Sectioning Aid System.
Thom
arrayworkshop.com
______________________________________________________________
From: "Thomas Pier" <tp2 <@t> medicine.wisc.edu>
To: <histonet <@t> lists.utsouthwestern.edu>, <thoward <@t> unm.edu>
Subject: Re: [Histonet] tape transfer paraffin question
Date: Mon, 26 Mar 2007 14:59:00 -0500
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>Sometimes those parts of the tape stick to the slide. As long as
the
>pink tape is gone, the area over the cores should be free of tape.
>
>Tom Pier
>
> >>> "Tamara A Howard" <thoward <@t> unm.edu> 03/26/07 2:17 PM >>>
>Help!
>
>I've been given 2 tissue array slides that were made using
>one of the tape transfer systems. I've never had tape
>slides - isn't the tape supposed to be removed before
>staining? The supplier of the slides says to just proceed
>as usual, but these look to me as if they still have tape,
>and I can see the "this side to adhesive" & "knife edge"
>(or whatever the other message is) - shouldn't those
>messages be gone if the tape has been removed? Maybe they
>are supposed to look this way, and I'm just being paranoid
>(they are ungodly expensive samples).
>
>Thanks!
>
>Tamara
>
>
>***************************
>Tamara Howard
>Cell Biology & Physiology
>UNM-HSC
>Albuquerque, NM
>***************************
>
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