[Histonet] Von Kossa

[Molinari, Betsy]

Ralph,

Thank you so much for your input. Now that I know it has been done and
with your advice I feel I can move forward with more confidence. Thanks
again.

 

 

Betsy Molinari HT (ASCP)

Texas Heart Institute

Cardiovascular Pathology

6770 Bertner Ave.

Houston,TX 77030

832-355-6524

832-355-6812 (fax)

 

-----Original Message-----
From: koellingr <@t> comcast.net [mailto:koellingr <@t> comcast.net] 
Sent: Friday, March 23, 2007 12:47 AM
To: Molinari, Betsy; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Von Kossa

 

Betsy,

In case you haven't had specific procedural reply's, I'll say yes.  Did
it for several years but back where I can't get at notes and 15 years of
memory loss.  Did VK on my cultures I maintained for project.  Performed
on 8-well tissue culture slides, both glass and plastic and tissue
culture plates of all sizes.  The procedure is basically the same as the
VK you know-it just needs a bit of inginuity. Anything plastic obviously
you can't go through xylenes but that didn't matter as after NFR, washed
in water, got a coverlip on somehow with aqueous media and photographed
right on plate with inverted scope or slide as usual.  Didn't use "the
sun" but a great lamp.  Make sure you have a good positive control to
work up procedure.  I made up my own pos and neg tissue culture controls
for VK as my experiments went forward. Look in PubMed and find van
Griensven M,  Exp Toxiicol Pathol  2002 Jul; 54 (1) 25-9.  Or the
Journal Calci fied Tissue International vol 72, Number 5, May 2003
537-547 "Von Kossa Staining alone is not sufficient to confirm that
mineralization in vitro represents bone formation;   the authors of
which (Bonewald, Harris, Rosser, Dallas)came out of Texas Health
Sciences Center down your way.  There are many other such references in
a PubMed search.  Would that they had published years earlier so I
wouldn't have had so many nights and weekends in the lab.  But my thesis
adviser honestly did not say there would be much glory and stardom, just
lots of hard work.

 

Raymond Koelling

Phenopath Laboratories

Seattle, WA

 

	-------------- Original message -------------- 
	From: "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu> 
	
	> Hi, 
	> 
	> Has anyone run a Von Kossa on a tissue culture? 
	> 
	> Thanks, 
	> 
	> Betsy 
	> 
	> 
	> 
	> 
	> 
	> Betsy Molinari HT (ASCP) 
	> 
	> Texas Heart Institute 
	> 
	> Cardiovascular Pathology 
	> 
	> 6770 Bertner Ave. 
	> 
	> Houston,TX 77030 
	> 
	> 832-355-6524 
	> 
	> 832-355-6812 (fax) 
	> 
	> 
	> 
	> _______________________________________________ 
	> Histonet mailing list 
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