[Histonet] Von Kossa

[koellingr <@t> comcast.net]

Betsy,
In case you haven't had specific procedural reply's, I'll say yes.  Did it for several years but back where I can't get at notes and 15 years of memory loss.  Did VK on my cultures I maintained for project.  Performed on 8-well tissue culture slides, both glass and plastic and tissue culture plates of all sizes.  The procedure is basically the same as the VK you know-it just needs a bit of inginuity. Anything plastic obviously you can't go through xylenes but that didn't matter as after NFR, washed in water, got a coverlip on somehow with aqueous media and photographed right on plate with inverted scope or slide as usual.  Didn't use "the sun" but a great lamp.  Make sure you have a good positive control to work up procedure.  I made up my own pos and neg tissue culture controls for VK as my experiments went forward. Look in PubMed and find van Griensven M,  Exp Toxiicol Pathol  2002 Jul; 54 (1) 25-9.  Or the Journal Calcified Tissue International vol 72, Number 5, May 2003 537-547
 "Von Kossa Staining alone is not sufficient to confirm that mineralization in vitro represents bone formation;   the authors of which (Bonewald, Harris, Rosser, Dallas)came out of Texas Health Sciences Center down your way.  There are many other such references in a PubMed search.  Would that they had published years earlier so I wouldn't have had so many nights and weekends in the lab.  But my thesis adviser honestly did not say there would be much glory and stardom, just lots of hard work.

Raymond Koelling
Phenopath Laboratories
Seattle, WA

-------------- Original message -------------- 
From: "Molinari, Betsy" <BMolinari <@t> heart.thi.tmc.edu> 

> Hi, 
> 
> Has anyone run a Von Kossa on a tissue culture? 
> 
> Thanks, 
> 
> Betsy 
> 
> 
> 
> 
> 
> Betsy Molinari HT (ASCP) 
> 
> Texas Heart Institute 
> 
> Cardiovascular Pathology 
> 
> 6770 Bertner Ave. 
> 
> Houston,TX 77030 
> 
> 832-355-6524 
> 
> 832-355-6812 (fax) 
> 
> 
> 
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